Osmotic and non-osmotic regulation of arginine vasopressin (AVP) release, mRNA, and promoter activity in small cell lung carcinoma (SCLC) cells

Molecular and Cellular Endocrinology
J K KimR W Schrier

Abstract

Arginine vasopression (AVP) is synthesized in the magnocellular neurons of the hypothalamus and stored in the posterior pituitary. It has been shown that hypothalamic AVP mRNA is increased during experimental stimulation of osmotic and non-osmotic stimulation of AVP release. The mechanisms underlying the stimulation of AVP biosynthesis in these conditions are not known. The present study was, therefore, performed to measure AVP release, AVP mRNA level, and AVP gene promoter activity during osmotic and non-osmotic stimulation of AVP secretion in the small cell lung carcinoma (SCLC) cells. AVP release was measured by radioimmunoassay, steady state levels of AVP mRNA by solution hybridization, and AVP gene promoter activity exhibited by a 1.5 kb 5'-flanking AVP gene fragment fused to a luciferase reporter after SCLC cells were subjected to osmotic or non-osmotic conditions. High media osmolality (330 mOsm) significantly increased AVP release (control (C) 1.42 +/- 0.27 vs. High Osm 3.67 +/- 0.39 pg/2 x 10(6) cells, N = 9, P < 0.002); AVP mRNA (C 173.6 +/- 16.8 vs. High Osm 280.1 +/- 19.4 pg/2 x 10(6) cells, N = 7, P < 0.001); and AVP gene promoter activity (C 1353 +/- 99 vs. High Osm 2026 +/- 134 L.U./10(-4) U beta-gal, N = 8, P < ...Continue Reading

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Citations

Jul 31, 1998·Lancet·S Kumar, T Berl
Dec 14, 2001·Biochemical and Biophysical Research Communications·J K KimR W Schrier
Nov 29, 2011·Annales d'endocrinologie·Marie-Christine VantyghemClaire Douillard
Nov 30, 2005·European Journal of Haematology·Theodosios D FilippatosMoses S Elisaf
May 5, 2000·Experimental Physiology·W G North
Dec 10, 1999·American Journal of Hematology·H J MilionisM S Elisaf
Jan 3, 2001·Microscopy Research and Technique·A Chodobski, J Szmydynger-Chodobska
Sep 2, 1999·British Journal of Cancer·J M CoulsonP J Woll
Jan 1, 1997·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Rodriguez, W Driever

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