PMID: 9172073Jun 1, 1997Paper

Osmotic 'detubulation' in frog muscle arises from a reversible vacuolation process

Journal of Muscle Research and Cell Motility
F A Gallagher, C L Huang

Abstract

Isolated Rana temporaria sartorius muscle fibres were subject to introduction and subsequent withdrawal of 400 mM extracellular glycerol, exposures to high divalent ion concentrations and then cooling. Tubular detachment was then assessed through changes in the action potential afterdepolarization. (1) The rapid (5-10 min) rather than slow cooling step (30 min) produced a gradual (30 min) development of detubulation arrested by the subsequent replacement of glycerol and reversed by addition of 350 mM sucrose. Such osmotic agents influenced neither resting potentials of intact or detubulated fibres nor action potentials in intact fibres. (2) Full tubular detachment was achieved by 40 min. Laser epifluorescence microscopy demonstrated an accompanying tubular vacuolation through its trapping of a Rhodamine dye. (3) Subsequent re-additions (at 10-80 min) of glycerol restored the afterdepolarization in 30% of detubulated fibres and correspondingly reduced vacuolation. Sustained (> 60 min) exposures to 350 mM sucrose, applied between 30-60 min, both reversed tubular isolation in 70% of detubulated fibres and abolished tubular vacuolation. Finally, results from transient (10-30 min) sucrose exposures resembled the consequences of sust...Continue Reading

Citations

Dec 25, 2003·The Journal of Physiology·Emily A FerencziChristopher L-H Huang
Aug 13, 1999·The American Journal of Physiology·M KawaiC H Orchard
Jan 15, 2005·The Journal of Physiology·James A FraserChristopher L-H Huang
Jan 13, 2006·Pflügers Archiv : European journal of physiology·Juliet A Usher-SmithChristopher L-H Huang
Jun 10, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Venkateshwar MutyamCarissa M Krane
Nov 19, 2004·Journal of Muscle Research and Cell Motility·Diana X-L ChinChristopher L-H Huang
Jul 11, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Keita UchidaAnatoli N Lopatin

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