PMID: 3969524Jan 1, 1985

Osmotic fragility of drug carrier erythrocytes

Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
U Sprandel, N Zöllner

Abstract

Erythrocytes have been proposed as biogradable cellular carriers for drugs. Potentials of this therapeutic approach are organ-specific targeting, protection and prolonged in vivo function of the encapsulated drugs. Previous studies demonstrated the advantage of a hypo-osmotic dialysis procedure for macromolecule loading resulting in cells that are closely similar to normal erythrocytes. Osmotic fragility of unloaded and loaded "carrier" erythrocytes was studied both in respect of shelf-life and in vivo survival. Sudden haemolysis which is characteristic for normal erythrocytes was never obtained with carrier erythrocytes. Haemolysis appeared at all osmotic pressures and increased stepwise indicating the existence of various cell populations. However, the majority of cells were haemolysed at lower values of the osmotic fragility curve. Osmotic fragility was highly increased when cytotoxic chemotherapeutics were encapsulated, and these cells appeared as spherocytes using scanning electron microscopy. Osmotic fragility proved to be a simple but reliable method for the in vitro evaluation of carrier erythrocytes and the effect of the encapsulated substances.

References

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Citations

Jan 1, 1990·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·U Sprandel
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·J R DeLoach, R Droleskey
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Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A De FloraE Zocchi
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A De FloraU Benatti
Jun 1, 1990·Artificial Organs·M A el-KalayS A al-Shirbini
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Pharmaceutical Preparations
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