Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells

PloS One
Moritz VeltmannAndreas Bringmann

Abstract

Although systemic hypertension is a risk factor of age-related macular degeneration, antihypertensive medications do not affect the risk of the disease. One condition that induces hypertension is high intake of dietary salt resulting in increased blood osmolarity. In order to prove the assumption that, in addition to hypertension, high osmolarity may aggravate neovascular retinal diseases, we determined the effect of extracellular hyperosmolarity on the expression of angiogenic cytokines in cultured human retinal pigment epithelial (RPE) cells. Hyperosmolarity was induced by the addition of 100 mM NaCl or sucrose to the culture medium. Hypoxia and oxidative stress were induced by the addition of the hypoxia mimetic CoCl2 and H2O2, respectively. Alterations in gene expression were determined with real-time RT-PCR. Secretion of bFGF was evaluated by ELISA. Cell viability was determined by trypan blue exclusion. Nuclear factor of activated T cell 5 (NFAT5) expression was knocked down with siRNA. Hyperosmolarity induced transcriptional activation of bFGF, HB-EGF, and VEGF genes, while the expression of other cytokines such as EGF, PDGF-A, TGF-β1, HGF, and PEDF was not or moderately altered. Hypoxia induced increased expression of t...Continue Reading

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Citations

Jul 19, 2019·Annals of the New York Academy of Sciences·Eszter FabianIstvan A Krizbai
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Hai-Fan YuBin Guo
Sep 16, 2016·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Anica WingesMargrit Hollborn
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Apr 26, 2019·Oxidative Medicine and Cellular Longevity·Elzbieta PawlowskaJanusz Blasiak
Aug 9, 2020·Frontiers in Endocrinology·Denes TothDora Reglodi
Feb 14, 2021·In Vitro Cellular & Developmental Biology. Animal·Sepideh Alinezhad-BermiHana Hanaee-Ahvaz
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pol Fernandez-GonzalezPere Garriga
Aug 4, 2021·Biochimica Et Biophysica Acta. General Subjects·Weiqi Li, Wen Wang
Nov 14, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jia LiXiaoling Liang

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Methods Mentioned

BETA
electrophoresis
PCR
ELISA
transfection

Software Mentioned

Prism

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