Osmotic lysis of tumor spill in ovarian cancer: a murine model

American Journal of Obstetrics and Gynecology
P C Morris, V Scholten

Abstract

Our purpose was to determine, in the murine model, whether human ovarian cancer cells injected intraperitoneally are subject to osmotic lysis by peritoneal lavage with sterile water, thereby decreasing the establishment of peritoneal implants. Preliminary experiments on six nude mice determined that the injection of 20 million cells of the SKOV-3 cell line reliably leads to the establishment of intraperitoneal tumor xenografts in the mice within 60 days. Four other nude mice functioned as sham controls undergoing peritoneal lavage with 3 to 4 ml of saline solution or sterile water to determine any adverse effects from the lavage alone. Subsequently, 36 nude (nu/nu) mice were injected intraperitoneally with 1 ml of the SKOV-3 cell line at a concentration of 20 million cells per milliliter. Alternate mice then underwent intraperitoneal lavage with either 3 to 4 ml of normal saline solution (control group) or sterile water (study group). The mice were followed up until tumor growth caused a moribund status or until 60 days after injection and then were killed. At necropsy the number and size of tumor nodules were recorded, and each mouse was assigned a composite tumor score. Statistical comparison used the X2 or Fisher's exact tes...Continue Reading

References

Dec 9, 1983·JAMA : the Journal of the American Medical Association·R C YoungJ P Smith
Sep 1, 1993·American Journal of Obstetrics and Gynecology·N C GleesonD Cavanagh
Jun 1, 1993·Journal of Endourology·D S GoldsteinL R Kavoussi

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Citations

May 19, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M CanisM A Bruhat
Apr 3, 2003·Cytokine·Kristan A KeyesEnrique Alvarez
Feb 9, 2011·Annals of Surgical Oncology·Fumito ItoJoseph J Skitzki
Dec 14, 2011·Interactive Cardiovascular and Thoracic Surgery·Teresa TsakokRobert Watson
May 16, 2006·The Journal of Urology·Ronney AbazaSteven H Selman
Jul 30, 2014·The Annals of Otology, Rhinology, and Laryngology·Kunal A LodhiaStuart C Winter
Feb 12, 2017·The Journal of Physiological Sciences : JPS·Atsushi ShiozakiEigo Otsuji

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