PMID: 3755760Jan 1, 1986Paper

Osmotic properties of human red cells

The Journal of Membrane Biology
A K SOLOMONJ A Dix

Abstract

When an osmotic pressure gradient is applied to human red cells, the volume changes anomalously, as if there were a significant fraction of "nonosmotic water" which could not serve as solvent for the cell solutes, a finding which has been discussed widely in the literature. In 1968, Gary-Bobo and Solomon (J. Gen. Physiol. 52:825) concluded that the anomalies could not be entirely explained by the colligative properties of hemoglobin (Hb) and proposed that there was an additional concentration dependence of the Hb charge (ZHb). A number of investigators, particularly Freedman and Hoffman (1979, J. Gen. Physiol. 74:157) have been unable to confirm Gary-Bobo and Solomon's experimental evidence for this concentration dependence of ZHb and we now report that we are also unable to repeat the earlier experiments. Nonetheless, there still remains a significant anomaly which amounts to 12.5 +/- 0.8% of the total isosmotic cell water (P much less than 0.0005, t test), even after taking account of the concentration dependence of the Hb osmotic coefficient and all the other known physical chemical constraints, ideal and nonideal. It is suggested that the anomalies at high Hb concentration in shrunken cells may arise from the ionic strength...Continue Reading

References

Jun 28, 1977·Biochemistry·E Strapazon, T L Steck
Sep 1, 1977·The Journal of General Physiology·J Brahm
Aug 1, 1979·The Journal of General Physiology·J C Freedman, J F Hoffman
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Citations

Sep 1, 1996·Journal of Cellular Physiology·W J Armitage, B K Juss
Jul 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M A MartinR L Armstrong
Nov 3, 1988·Biochimica Et Biophysica Acta·M MosiorJ Gomułkiewicz
Dec 12, 1991·Biochimica Et Biophysica Acta·B Sarkadi, J C Parker
Aug 5, 2008·Cryobiology·Richelle C PrickettLocksley E McGann
Jan 15, 2008·Cryobiology·Corinna M Kashuba BensonJohn K Critser
Jul 26, 2005·Journal of Theoretical Biology·Pierre Wong
Jan 13, 2015·Computational and Mathematical Methods in Medicine·Heyuan QiaoDayong Gao
Apr 15, 2015·The Journal of General Physiology·Olaf S Andersen
Apr 16, 1998·Journal of Applied Physiology·J M Karch, J S Lee
Jan 1, 1990·Critical Reviews in Oncology/hematology·T ShigaK Kon

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