PMID: 9430620Feb 28, 1998Paper

Osmotic sensitivity of Ca2+ and H+ transporters in corn roots: effect on fluxes and their oscillations in the elongation region

The Journal of Membrane Biology
Sergey Shabala, I A Newman

Abstract

Seedling roots of corn were treated with different concentrations of mannitol-containing solution for 1 to 1.5 hr, and net fluxes of Ca2+ and H+ were measured in the elongation region. H+ fluxes were much more sensitive to osmotic pressure than were Ca2+ fluxes. Oscillations of 7-min period in H+ flux, normally observed in the control, were almost fully suppressed at high osmotic concentrations. Net H+ flux was shifted from average efflux of 25 +/- 3 nmol m-2 sec-1 to average influx of 10 +/- 5 nmol m-2 sec-1 after the incubation in 100 mm mannitol. The larger the osmotic concentration, the larger was the H+ influx. This flux caused the unbuffered solution of pH 4.85 to change to pH 5.3 after mannitol application. It appears that the osmoticum suppresses oscillatory H+ extrusion at the plasma membrane. Discrete Fourier Transforms of the H+ flux data showed that, apart from suppression of the 7-min oscillations in H+ flux, mannitol also promoted the appearance of faster 2-min oscillations. Ca2+ influx slightly increased after mannitol treatment. In addition the 7-min oscillatory component of Ca2+ flux remained apparent thereby showing independence of H+ flux.

Citations

Jun 12, 2003·FEMS Microbiology Letters·Myriam Duckely, Barbara Hohn
May 30, 2002·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·D James MorreDorothy M Morre
Jun 20, 2015·The Plant Journal : for Cell and Molecular Biology·Suli YanYingbai Shen
Oct 1, 2011·Biomolecular Concepts·Sergey Shabala, Lana Shabala
Jan 25, 2011·Journal of Plant Physiology·Maheswari JayakannanZed Rengel
Jul 20, 2012·Journal of Bioinformatics and Computational Biology·Irina VolokhinaMikhail Chumakov
Feb 27, 2017·Computational Biology and Chemistry·Irina VolokhinaMikhail Chumakov
Aug 11, 2000·Journal of Experimental Botany·S ShabalaI Newman
Jul 16, 2011·Chemical Society Reviews·Eric S McLamore, D Marshall Porterfield

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