Osmotic shock induces structural damage on equine spermatozoa plasmalemma and mitochondria

Theriogenology
L González-FernándezB Macías-García

Abstract

The present study aimed to elucidate the effects that osmotic shock exerts on equine spermatozoa. To achieve this goal, a retrospective study of the cellular volume of 40 equine ejaculates subjected to osmolarities ranging from 75 to 900 mOsm in Biggers-Whitten-Whittingham (BWW) media was performed using a Multisizer3 Coulter Counter®. The 300 mOsm BWW solution was used as control. The sperm volume ranged between 37.93±0.6 (mean±Standard Error of the Mean (SEM)) in 75 mOsm BWW to 21.61±0.27 (mean±SEM) for 900 mOsm BWW. Thus the spermatozoa behaved as linear osmometers when adjusted to the Boyle Van't Hoff equation (R2=0.9808). After the different osmotic challenges, spermatozoa were returned to 300 mOsm BWW and the cellular volume was measured again. The results showed that the spermatozoa were able to retrieve the isosmolar volume (20.81±0.34; mean±SEM). Also, an ultrastructural study of spermatozoa membrane and mitochondria was accomplished using Transmission Electron Microscopy (TEM) after the osmotic challenges in 2 ejaculates. As observed by TEM, sperm plasmalemma swelled and detached from the sperm head in hypotonic conditions (75 mOsm), with blebbing on return to isosmolarity. When subjected to 900 mOsm, the sperm plasma...Continue Reading

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Citations

Oct 4, 2014·Animal Reproduction Science·S Gomes-AlvesP de Paz
Nov 26, 2015·Reproduction in Domestic Animals = Zuchthygiene·C M Balao da SilvaF J Peña
Apr 27, 2012·Animal Reproduction Science·B Macías-GarcíaJ M Morrell
Sep 19, 2015·Reproduction in Domestic Animals = Zuchthygiene·H SiemeW F Wolkers
Jun 3, 2015·Reproduction in Domestic Animals = Zuchthygiene·F J PeñaC Balao da Silva
May 13, 2014·The International Journal of Biochemistry & Cell Biology·Noah MoruzziKerstin Brismar
Feb 13, 2018·Reproduction in Domestic Animals = Zuchthygiene·H S MartinsM A Lagares
Oct 20, 2018·Anatomia, histologia, embryologia·Lucia OlexikovaPeter Chrenek
Sep 13, 2019·Reproduction in Domestic Animals = Zuchthygiene·Stuart MeyersAzarene Foutouhi
Nov 17, 2020·Frontiers in Veterinary Science·Renato ZampiniSilvana A Apichela

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