PMID: 9425243Mar 14, 1998Paper

Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis

Current Microbiology
S M RuzalC Sanchez-Rivas

Abstract

In Bacillus subtilis, osmotolerance is a stationary phase-dependent, adaptive response inhibiting sporulation and sharing common regulators with this process. The extent of this inhibition was determined by measuring transcription activity of promoter lacZ fusions to early sigma genes (spoIIG and spoIIA coding for precursors of sigmaE and sigmaF) and to reporters of them (spoIID, spoIIQ and spoIIIG), in the absence and presence of 0.6 M or 1 M NaCl. The transcription activity of these sigma precursors, normally occurring at the onset of the stationary phase, was reduced to 30-50% of their maximal expression in hyperosmotic conditions; expression of genes under their control was, however, more inhibited (<10%). Therefore, sporulation was blocked at the sigma sigmaE and sigmaF activation steps. This assumption was confirmed by electron microscopic examinations of hyperosmotic cultures, which presented asymmetric septa characteristic of stage II mutants. Discussion was focused on the particular composition and/or structure of membranes during hyperosmotic growth and their involvement in the arrest of sporulation.

Citations

May 3, 2000·International Journal of Food Microbiology·C S LópezE Rivas
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J M WoodL T Smith
Dec 22, 2004·Journal of Applied Microbiology·M PiuriS M Ruzal
Sep 7, 2001·Journal of Bacteriology·A PetersohnM Hecker
Jan 16, 2002·Journal of Bacteriology·Tamara HoffmannErhard Bremer
Dec 10, 2008·Research in Microbiology·María Mercedes PalominoSandra M Ruzal
Jun 24, 2004·Biotechnology and Bioengineering·Susmita SahooG K Suraishkumar
Mar 4, 2006·Microbiology·Claudia S LópezCarmen Sánchez-Rivas

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