Osmotic stress changes carbohydrate partitioning and fructose-2,6-bisphosphate metabolism in barley leaves

Functional Plant Biology : FPB
Dorthe VilladsenT H Nielsen

Abstract

Carbohydrate metabolism was investigated in barley leaves subjected to drought or osmotic stress induced by sorbitol incubation. Both drought and osmotic stress resulted in accumulation of hexoses, depletion of sucrose and starch, and 5-10-fold increase in the level of the regulatory metabolite fructose-2,6-bisphosphate (Fru-2,6-P2). These changes were paralleled by an increased activity ratio of fructose-6-phosphate,2-kinase / fructose-2,6-bisphosphatase (F2KP). The drought-induced changes in carbohydrate content and Fru-2,6-P2 metabolism were reversed upon re-watering. This reveals a reversible mechanism for modification of the F2KP enzyme activity. This suggests that F2KP might be involved in altering carbohydrate metabolism during osmotic stress. However, labelling with [14C]-CO2 showed that sucrose synthesis was not inhibited, despite the increased Fru-2,6-P2 levels, and demonstrated that increased flux into the hexose pools probably derived from sucrose hydrolysis. Similar effects of osmotic stress were observed in leaf sections incubated in the dark, showing that the changes did not result from altered rates of photosynthesis. Metabolism of [14C]-sucrose in the dark also revealed increased flux into hexoses and reduced f...Continue Reading

References

May 1, 1989·Archives of Biochemistry and Biophysics·J L HuberT H Nielsen
Sep 7, 2001·Trends in Plant Science·F A HoekstraJ Buitink
Feb 22, 2002·European Journal of Biochemistry·Jonathan E Markham, Nicholas J Kruger
Feb 12, 2004·The Plant Journal : for Cell and Molecular Biology·Anna KulmaCarol MacKintosh
Oct 27, 2004·Trends in Plant Science·Tom Hamborg NielsenDorthe Villadsen
Feb 1, 2004·Functional Plant Biology : FPB·Danica E Goggin, Tim L Setter
Aug 1, 2004·Functional Plant Biology : FPB·Stephen J TrevanionJohn E Lunn
Nov 1, 2004·Functional Plant Biology : FPB·Marta S LopesJosé L Araus
Mar 1, 2003·Functional Plant Biology : FPB·Manuela M ChavesJoão S Pereira

❮ Previous
Next ❯

Citations

Jun 3, 2009·Journal of Experimental Botany·Mikhail A Semenov, Nigel G Halford
Sep 1, 2006·Functional Plant Biology : FPB·Yong LiuAimin Zhang
Jan 31, 2012·Photosynthesis Research·Usue Pérez-LópezAlberto Muñoz-Rueda
Mar 1, 2009·Functional Plant Biology : FPB·Christophe ValtaudAndrée Bourbouloux
Jan 15, 2010·Plant Biotechnology Journal·Victoria J Maloney, Shawn D Mansfield
Mar 10, 2017·The New Phytologist·Matthias Thalmann, Diana Santelia
Nov 28, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pallavi Mishra, R S Dubey
Mar 12, 2021·Plant Physiology and Biochemistry : PPB·Pooja Moni BaruahNiraj Agarwala

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.