Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress-Related Gene Expression

Frontiers in Plant Science
Liting ZhangTongbao Lin

Abstract

Although, tauroursodeoxycholic acid (TUDCA) has been widely studied in mammalian cells because of its role in inhibiting apoptosis, its effects on plants remain almost unknown, especially in the case of crops such as wheat. In this study, we conducted a series of experiments to explore the effects and mechanisms of action of TUDCA on wheat growth and cell death induced by osmotic stress. Our results show that TUDCA: (1) ameliorates the impact of osmotic stress on wheat height, fresh weight, and water content; (2) alleviates the decrease in chlorophyll content as well as membrane damage caused by osmotic stress; (3) decreases the accumulation of reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes under osmotic stress; and (4) to some extent alleviates osmotic stress-induced cell death probably by regulating endoplasmic reticulum (ER) stress-related gene expression, for example expression of the basic leucine zipper genes bZIP60B and bZIP60D, the binding proteins BiP1 and BiP2, the protein disulfide isomerase PDIL8-1, and the glucose-regulated protein GRP94. We also propose a model that illustrates how TUDCA alleviates osmotic stress-related wheat cell death, which provides an important theoretical bas...Continue Reading

References

Jan 1, 1984·Methods in Enzymology·H Aebi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 10, 2002·Annals of Botany·H Griffiths, M A J Parry
Oct 18, 2002·Journal of Cellular Physiology·Carlo TuranoAnna Ferraro
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marieke H SchoemakerHan Moshage
Mar 1, 1977·Plant Physiology·T Brennan, C Frenkel
Nov 28, 2007·The Journal of Biological Chemistry·Naohide Watanabe, Eric Lam
Apr 26, 2008·Investigative Ophthalmology & Visual Science·M Joe PhillipsMachelle T Pardue
Jan 30, 2010·Science·H Charles J GodfrayCamilla Toulmin
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brett WilliamsMartin B Dickman
Oct 29, 2010·Plant & Cell Physiology·Taishi UmezawaKazuko Yamaguchi-Shinozaki
May 21, 2011·Cell Death and Differentiation·T IshikawaE Lam
Jan 19, 2012·Nature Reviews. Molecular Cell Biology·Claudio Hetz
May 31, 2012·Frontiers in Plant Science·Silvia Hüttner, Richard Strasser
Sep 4, 2012·International Journal of Molecular Sciences·Si-Jia WangYong-Bin Yan
Dec 10, 2013·Plant Physiology·Humberto H CarvalhoElizabeth P B Fontes
Mar 5, 2014·Frontiers in Plant Science·Yao-Min CaiPatrick Gallois
Mar 13, 2014·Frontiers in Plant Science·Renu SrivastavaStephen H Howell
Apr 8, 2014·Antioxidants & Redox Signaling·Stewart Siyan Cao, Randal J Kaufman
Jun 14, 2014·Frontiers in Plant Science·Brett WilliamsMartin B Dickman
Jun 26, 2014·The Plant Journal : for Cell and Molecular Biology·Zheng-Ting YangJian-Xiang Liu
Mar 6, 2015·Frontiers in Plant Science·Veselin PetrovTsanko S Gechev
Oct 28, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·A Kobylińska, Małgorzata M Posmyk

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Methods Mentioned

BETA
environmental stresses
protein folding
PCR
MDA
genetic-modification
environmental stress

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis