Osmotic Stress Induced Desorption of Calcium Ions from Dipolar Lipid Membranes

Langmuir : the ACS Journal of Surfaces and Colloids
Lea FinkUri Raviv

Abstract

The interaction between multivalent ions and lipid membranes with saturated tails and dipolar (net neutral) headgroups can lead to adsorption of the ions onto the membrane. The ions charge the membranes and contribute to electrostatic repulsion between them, in a similar manner to membranes containing charged lipids. Using solution X-ray scattering and the osmotic stress method, we measured and modeled the pressure-distance curves between partially charged membranes containing mixtures of charged (1,2-dilauroyl-sn-glycero-3-phospho-l-serine, DLPS) and dipolar (1,2-dilauroyl-sn-glycero-3-phosphocholine, DLPC) lipids over a wide range of membrane charge densities. We then compared these pressure-distance curves with those of DLPC membranes in the presence of 10 mM CaCl2. Our data and modeling show that when low osmotic stress is applied to the DLPC bilayers, the membrane charge density is equivalent to that of a charged membrane containing ca. 4 mol % DLPS and 96 mol % DLPC. As the osmotic stress increased, the charge density of the DLPC membrane decreased and resembled that of a membrane containing ca. 1 mol % DLPS. These data are consistent with desorption of the calcium ions from the DLPC membrane with increasing osmotic stress.

References

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Nov 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Or SzekelyUri Raviv
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Jan 14, 2016·The Journal of Physical Chemistry. B·Clayton B CasperHeather Cecile Allen

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Citations

May 22, 2018·Physical Chemistry Chemical Physics : PCCP·Soumya Kanti DeAnjan Chakraborty
Jul 10, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Lea FinkUri Raviv
Sep 25, 2018·Biochemistry·Asaf ShemeshUri Raviv
Apr 16, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Soumya Kanti DeAnjan Chakraborty
Aug 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Lea FinkUri Raviv
Feb 26, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Niki Baccile, Viviana Cristiglio

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