PMID: 9445370Mar 7, 1998Paper

Osmotic stress induces variation in cellular levels of ornithine decarboxylase-antizyme

The Biochemical Journal
J L MitchellC Y Choe

Abstract

The polyamines, and especially putrescine, play an integral role in the physiological response of cells to varying extracellular osmotic conditions. Ornithine decarboxylase (ODC) synthesis and stability, as well as the activity of the polyamine transporter, had all been reported to be very sensitive to media osmolarity in different cells and tissues, yet the mechanism of this complex, co-ordinated response was not known. In this study we have determined that all these aspects of osmotic-shock response may be mediated by the common regulatory protein, ODC-antizyme. HTC cells were induced for antizyme and then exposed to media of reduced osmotic strength. Both antizyme activity and protein decreased rapidly, under these conditions, to new steady-state levels that depended upon the degree of reduction in media tonicity. This antizyme reduction was found to be due to a rapid increase in antizyme degradation, with a half-life decrease from 75 min down to 45 min occurring immediately upon exchanging media. In complementary experiments, increased media tonicity induced elevated antizyme levels and stability. The sensitivity of antizyme turnover to osmotic conditions was also observed in DH23b cells, which contain elevated levels of mo...Continue Reading

Citations

Aug 28, 2003·The Journal of Biological Chemistry·Noriyuki MuraiSenya Matsufuji
Nov 3, 2005·The Journal of Biological Chemistry·Kenji HoshinoKazuei Igarashi
Apr 29, 2009·Cellular and Molecular Life Sciences : CMLS·Chaim Kahana
Jan 19, 2012·Fish Physiology and Biochemistry·Charles A BrownChristopher C Green
Feb 15, 2001·Journal of Cellular Physiology·E MatteucciM A Desiderio
Sep 1, 2007·Journal of Cellular and Molecular Medicine·H J RheeJ K Lee
Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·Q YuanL R Johnson
Aug 2, 2007·Physiological Genomics·Svetlana KalujnaiaGordon Cramb
Feb 27, 2001·Journal of Neuroscience Research·S M YatinD A Butterfield
Mar 4, 2021·Molecular Ecology·Molly A AlbeckerMichael W McCoy
Oct 24, 2002·Biochimica Et Biophysica Acta·Tomasz HascilowiczYasuko Murakami

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