Osmotic stress response: quantification of cell maintenance and metabolic fluxes in a lysine-overproducing strain of Corynebacterium glutamicum

Applied and Environmental Microbiology
Cristian A VarelaEduardo Agosin

Abstract

Osmotic stress diminishes cell productivity and may cause cell inactivation in industrial fermentations. The quantification of metabolic changes under such conditions is fundamental for understanding and describing microbial behavior during bioprocesses. We quantified the gradual changes that take place when a lysine-overproducing strain of Corynebacterium glutamicum is grown in continuous culture with saline gradients at different dilution rates. The use of compatible solutes depended on environmental conditions; certain osmolites predominated at different dilution rates and extracellular osmolalities. A metabolic flux analysis showed that at high dilution rates C. glutamicum redistributed its metabolic fluxes, favoring energy formation over growth. At low dilution rates, cell metabolism accelerated as the osmolality was steadily increased. Flexibility in the oxaloacetate node proved to be key for the energetic redistribution that occurred when cells were grown at high dilution rates. Substrate and ATP maintenance coefficients increased 30- and 5-fold, respectively, when the osmolality increased, which demonstrates that energy pool management is fundamental for sustaining viability.

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Citations

Dec 7, 2010·Journal of Industrial Microbiology & Biotechnology·Meghna Rajvanshi, K V Venkatesh
Aug 16, 2003·European Journal of Biochemistry·Maria I KlapaGregory Stephanopoulos
Oct 4, 2007·Microbial Cell Factories·Vicente BernalJosé L Iborra
Mar 20, 2014·Microbial Cell Factories·Arul M VarmanYinjie J Tang
Apr 26, 2013·The Journal of Biological Chemistry·José M PastorManuel Cánovas
Jul 29, 2008·Metabolic Engineering·Curt R FischerGregory Stephanopoulos
Nov 6, 2008·Biotechnology and Bioengineering·Kjeld Raunkjaer Kjeldsen, Jens Nielsen
Dec 30, 2014·Journal of Biotechnology·Anne SteinkämperRichard Biener
Jan 26, 2005·FEMS Microbiology Letters·Srinivas Reddy PallerlaSiegfried M Schoberth
Aug 9, 2006·Biotechnology and Bioengineering·M CánovasJ L Iborra
Aug 1, 2018·Bioprocess and Biosystems Engineering·Yuewen SuJian Wang
Apr 13, 2021·Frontiers in Cellular and Infection Microbiology·Gabriel Moura MascarinChristopher A Dunlap

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