PMID: 11341979May 9, 2001Paper

Osmotically induced cytosolic free Ca(2+) changes in human neutrophils

Biochimica Et Biophysica Acta
M R MorrisM B Hallett

Abstract

Cytosolic free Ca(2+) concentration in neutrophils was measured by ratiometric fluorometry of intracellular fura2. Increasing the extracellular osmolarity, by either NaCl (300-600 mM) or sucrose (600-1200 mM), caused a rise in cytosolic free Ca(2+) (Delta(max) approximately equal to 600 nM). This was not due to cell lysis as the cytosolic free Ca(2+) concentration was reversed by restoration of isotonicity and a second rise in cytosolic free Ca(2+) could be provoked by repeating the change in extracellular osmolarity. Furthermore, the rise in cytosolic free Ca(2+) concentration occurred in the absence of extracellular Ca(2+), demonstrating that release of intracellular fura2 into the external medium did not occur. The osmotically-induced rise in cytosolic free Ca(2+) was not inhibited by either the phospholipase C-inhibitor U73122, or the microfilament inhibitor cytochalasin B, suggesting that neither signalling via inositol tris-phosphate or the cytoskeletal system were involved. However, the rise in cytosolic free Ca(2+) may have resulted from a reduction in neutrophil water volume in hyperosmotic conditions. As these rises in cytosolic Ca(2+) (Delta(max) approximately equal to 600 nM) were large enough to provoke changes in ...Continue Reading

References

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Citations

Sep 5, 2007·Cell and Tissue Research·Harriet NilssonGodfried M Roomans
Mar 31, 2012·Apoptosis : an International Journal on Programmed Cell Death·Katsuya DezakiYasunobu Okada
Oct 25, 2002·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·Evangelia DaviskasP T P Bye
Sep 24, 2005·Clinical and Experimental Immunology·M R MorrisM B Hallett

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