Osmotically induced shape changes of large unilamellar vesicles measured by dynamic light scattering

Biophysical Journal
J PencerF R Hallett

Abstract

Static and dynamic light scattering measurements have been used to characterize the size, size distribution, and shape of extruded vesicles under isotonic conditions. Dynamic light scattering was then used to characterize osmotically induced shape changes by monitoring changes in the hydrodynamic radius (R(h)) of large unilamellar vesicles (LUVs). These changes are compared to those predicted for several shapes that appear in trajectories through the phase diagram of the area difference elasticity (ADE) model (. Phys. Rev. E. 52:6623-6634). Measurements were performed on dioleoylphosphatidylcholine (DOPC) vesicles using two membrane-impermeant osmolytes (NaCl and sucrose) and a membrane-permeant osmolyte (urea). For all conditions, we were able to produce low-polydispersity, nearly spherical vesicles, which are essential for resolving well-defined volume changes and consequent shape changes. Hyper-osmotic dilutions of DOPC vesicles in urea produced no change in R(h), whereas similar dilutions in NaCl or sucrose caused reductions in vesicle volume resulting in observable changes to R(h). Under conditions similar to those of this study, the ADE model predicts an evolution from spherical to prolate then oblate shapes on increasing...Continue Reading

References

Apr 1, 1991·Journal of Electron Microscopy Technique·F R HallettP H Krygsman
Jan 30, 1991·Biochimica Et Biophysica Acta·R C MacDonaldL R Hu
Jan 1, 1990·Journal of Electron Microscopy Technique·Y TalmonD P Siegel
Apr 1, 1993·Chemistry and Physics of Lipids·S KölchensD F O'Brien
Feb 1, 1993·Biophysical Journal·B L MuiT D Madden
Dec 1, 1995·Trends in Biotechnology·G Gregoriadis
Nov 1, 1996·Biophysical Journal·G WhiteF R Hallett
Jun 1, 1994·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·L MiaoH G Döbereiner
Dec 1, 1995·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·M JaricM Wortis
Apr 8, 1999·European Biophysics Journal : EBJ·A J JinR Nossal
Jun 27, 2000·Biophysical Journal·W RawiczE Evans
Mar 1, 1999·Journal of Biological Physics·L Xu, H G Döbereiner

❮ Previous
Next ❯

Citations

Apr 15, 2006·European Biophysics Journal : EBJ·M A KiselevR H H Neubert
Jan 1, 2004·Journal of Biological Physics·Shaoxin LiJunfeng Li
Jul 30, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Jia-Yu WangKa Yee C Lee
Sep 21, 2013·ACS Nano·Romain SalvaSébastien Lecommandoux
Sep 8, 2006·The European Physical Journal. E, Soft Matter·J SabínF Sarmiento
Oct 4, 2011·BMC Plant Biology·Erika Kovács-BogdánBettina Bölter
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paula A GiardiniJulie A Theriot
Apr 27, 2010·Contrast Media & Molecular Imaging·Enzo TerrenoSilvio Aime
Apr 2, 2003·Biophysical Journal·Fuyuki TokumasuJames A Dvorak
Apr 7, 2015·Biochimica Et Biophysica Acta·Dominika WrobelJan Maly
Mar 25, 2014·Journal of Microencapsulation·Joy WolframHaifa Shen
Nov 13, 2013·Colloids and Surfaces. B, Biointerfaces·Joy WolframMauro Ferrari
Feb 8, 2005·Biophysical Journal·Giuseppe MaulucciGiuseppe Briganti
Oct 21, 2005·Analytical Biochemistry·Aleksandar VojtaEnrico Schleiff
Sep 24, 2016·The Journal of Physical Chemistry. B·Ravi BhattaraiAmiya Kumar Panda
Nov 23, 2005·Journal of Colloid and Interface Science·Matthew L LynchMichael R Weaver
Jun 27, 2014·Nature Communications·Mihail BarboiuThomas Fyles
Jan 11, 2019·Physics in Medicine and Biology·Dennis MüllerAlwin Kienle
Jan 15, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Richard G MorrisAlan J McKane
Jul 30, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Richard G Morris, Alan J McKane
Jan 1, 2016·Physical Biology·Erwan BiganStéphane Douady
Nov 15, 2020·Angewandte Chemie·Wenbo ZhangPeter Fratzl
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Juan SabínFélix Sarmiento
Apr 25, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Agnes CsiszárRudolf Merkel
Nov 8, 2008·The Journal of Physical Chemistry. B·Francesca Baldelli BombelliPiero Baglioni
Apr 29, 2021·Soft Matter·Rasangi M PereraGerald J Schneider
Mar 20, 2019·Journal of Pharmaceutical Sciences·Iren Yeeling WuMassimiliano Pio di Cagno
Aug 8, 2021·Acta Biomaterialia·Natalia OnishchenkoElena Vodovozova
Oct 4, 2012·Journal of the American Chemical Society·Carlos R López-BarrónNorman J Wagner
May 2, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Tao ZhuYuqiang Ma
May 24, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Martin LoewRobin L McCarley
Dec 5, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Jurriaan J J GillissenNam-Joon Cho
Feb 28, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Minmin Zhang, Serge G Lemay
May 17, 2018·ACS Applied Materials & Interfaces·Sara Garcia-SalinasVictor Sebastian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved