OsMYB80 Regulates Anther Development and Pollen Fertility by Targeting Multiple Biological Pathways

Plant & Cell Physiology
Xiaoying PanXiaoyan Tang

Abstract

Pollen development is critical to the reproductive success of flowering plants, but how it is regulated is not well understood. Here, we isolated two allelic male-sterile mutants of OsMYB80 and investigated how OsMYB80 regulates male fertility in rice. OsMYB80 was barely expressed in tissues other than anthers, where it initiated the expression during meiosis, reached the peak at the tetrad-releasing stage and then quickly declined afterward. The osmyb80 mutants exhibited premature tapetum cell death, lack of Ubisch bodies, no exine and microspore degeneration. To understand how OsMYB80 regulates anther development, RNA-seq analysis was conducted to identify genes differentially regulated by OsMYB80 in rice anthers. In addition, DNA affinity purification sequencing (DAP-seq) analysis was performed to identify DNA fragments interacting with OsMYB80 in vitro. Overlap of the genes identified by RNA-seq and DAP-seq revealed 188 genes that were differentially regulated by OsMYB80 and also carried an OsMYB80-interacting DNA element in the promoter. Ten of these promoter elements were randomly selected for gel shift assay and yeast one-hybrid assay, and all showed OsMYB80 binding. The 10 promoters also showed OsMYB80-dependent inducti...Continue Reading

References

Nov 14, 1997·The Plant Journal : for Cell and Molecular Biology·M G AartsA Pereira
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M YangH Ma
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Aiming WangGopalan Selvaraj
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·Jeremy PrestonRoger W Parish
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Xiaoyun DongDesh Pal S Verma
Sep 6, 2005·The Plant Cell·Ki-Hong JungGynheung An
Sep 19, 2006·Plant Physiology·Kirk E FrancisGregory P Copenhaver
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Jul 2, 2010·Journal of Integrative Plant Biology·Zhenhua LiuDabing Zhang
Nov 26, 2010·Science China. Life Sciences·Jun ZhuZhongNan Yang
Feb 1, 2011·Annual Review of Plant Biology·Tohru Ariizumi, Kinya Toriyama
Sep 21, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Dabing ZhangLu Zhu
Oct 1, 2011·Journal of Integrative Plant Biology·Jun ZhuZhong-Nan Yang
Nov 10, 2011·Biochimica Et Biophysica Acta·Michael B Prouse, Malcolm M Campbell
Nov 17, 2011·Plant Molecular Biology·Huy A PhanRoger W Parish

❮ Previous
Next ❯

Citations

Mar 26, 2021·Frontiers in Plant Science·Adil AbbasLiyong Cao
Sep 10, 2020·Plant Science : an International Journal of Experimental Plant Biology·Fernanda ValandroAlexandro Cagliari
Aug 8, 2021·International Journal of Molecular Sciences·Yilin JiangXiangyuan Wan
Oct 6, 2021·Scientific Reports·Muhammad-Redha Abdullah-ZawawiZeti-Azura Mohamed-Hussein

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP058039
SRP196452

Methods Mentioned

BETA
transgenic
reverse transcription PCR
transmission electron microscopy
RNA-Seq
affinity
affinity purification
DAP-seq
electrophoretic mobility shift assay
one-hybrid
ubiquitination

Software Mentioned

BlastP
KEGG
SIMM
AgriGO
Simultaneous Identification of Multiple Causal Mutations ( SIMM )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.