Osmylated DNA, a novel concept for sequencing DNA using nanopores

Nanotechnology
Anastassia Kanavarioti

Abstract

Saenger sequencing has led the advances in molecular biology, while faster and cheaper next generation technologies are urgently needed. A newer approach exploits nanopores, natural or solid-state, set in an electrical field, and obtains base sequence information from current variations due to the passage of a ssDNA molecule through the pore. A hurdle in this approach is the fact that the four bases are chemically comparable to each other which leads to small differences in current obstruction. 'Base calling' becomes even more challenging because most nanopores sense a short sequence and not individual bases. Perhaps sequencing DNA via nanopores would be more manageable, if only the bases were two, and chemically very different from each other; a sequence of 1s and 0s comes to mind. Osmylated DNA comes close to such a sequence of 1s and 0s. Osmylation is the addition of osmium tetroxide bipyridine across the C5-C6 double bond of the pyrimidines. Osmylation adds almost 400% mass to the reactive base, creates a sterically and electronically notably different molecule, labeled 1, compared to the unreactive purines, labeled 0. If osmylated DNA were successfully sequenced, the result would be a sequence of osmylated pyrimidines (1),...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 5, 1977·Journal of Molecular Biology·M D ColeM Beer
Jan 1, 1992·Methods in Enzymology·E Palecek
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J KasianowiczD W Deamer
Jul 13, 2001·Nature·J LiJ A Golovchenko
Mar 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·M BEER, MOUDRIANAKISEN
Nov 14, 1964·Nature·E N MOUDRIANAKIS, M BEER
Dec 8, 2006·Nucleic Acids Symposium Series·Kazuki TainakaAkimitsu Okamoto
Oct 11, 2008·Nature Biotechnology·Jay Shendure, Hanlee Ji
Oct 11, 2008·Nature Biotechnology·Daniel BrantonJeffery A Schloss
May 16, 2009·Journal of the American Chemical Society·Vinciane BorsenbergerStefan Howorka
Feb 26, 2011·Analytical and Bioanalytical Chemistry·Pavel KostečkaMiroslav Fojta
Apr 12, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jun ZhangGenfa Zhang
Jun 24, 2011·Journal of Applied Genetics·Chandra Shekhar PareekAndrzej Tretyn
Nov 10, 2011·Biophysical Journal·Binquan LuanGustavo Stolovitzky
Jan 31, 2012·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Emil PalečekMojmír Trefulka
Mar 27, 2012·Nature Biotechnology·Elizabeth A ManraoJens H Gundlach
Jun 5, 2012·Physics of Life Reviews·Meni Wanunu
Jul 26, 2012·Journal of Biomedicine & Biotechnology·Lin LiuMaggie Law
Oct 11, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·David C BellWilliam R Glover
Nov 20, 2012·Chemical Reviews·Joseph E ReinerJohn J Kasianowicz
Dec 4, 2012·The HUGO Journal·Sowmiya MoorthieCaroline F Wright
Dec 20, 2012·ACS Nano·Shouvik BanerjeeRashid Bashir
Feb 5, 2013·Nature Biotechnology·Jeff NivalaMark Akeson
Jul 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Slaven GarajDaniel Branton
Mar 25, 2014·PloS One·Harold KwokVincent Tabard-Cossa
Jan 1, 2010·Genes·Matthew W Anderson, Iris Schrijver
Jun 26, 2014·Nature Biotechnology·Andrew H LaszloJens H Gundlach
Sep 5, 2014·Molecular Ecology Resources·Alexander S Mikheyev, Mandy M Y Tin

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Citations

Oct 4, 2019·Scientific Reports·Madiha Sultan, Anastassia Kanavarioti
Aug 20, 2016·Bioconjugate Chemistry·Lyudmil Tserovski, Mark Helm

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