OspE2 of Shigella sonnei is required for the maintenance of cell architecture of bacterium-infected cells.

Infection and Immunity
Masashi MiuraHaruo Watanabe

Abstract

The OspE2 product of Shigella spp., the expression of which is regulated by the mxiE gene, is secreted through a type III secretion system into host cells. We investigated the function of OspE2 of Shigella sonnei by using cultured epithelial cells. Cells invaded by an ospE2 deletion mutant altered their morphology into the rounding shape, which was not due to cell death, whereas cells invaded by the wild-type strain kept their cell shape intact. The ospE2 mutation did not affect initial cell entry and multiplication in cells, but the mutant formed smaller-than-normal plaques on cell monolayers, indicating a deficiency in cell-to-cell spread by the bacteria. An mxiE deletion mutant also showed changes in cell morphology and deficiency in bacterial spread to adjacent cells. In cells invaded by the ospE2 mutant, disturbance of actin stress fibers was prominent at 3 h after invasion. Analysis of OspE2 localization indicated that the OspE2 protein accumulated on focal contact-like structures in the infected host cells. These results suggest that colocalization of the OspE2 protein in the focal contacts of infected cells may function to maintain an intact cell morphology. The morphological change induced by invasion of the ospE2 muta...Continue Reading

References

Jul 9, 1992·Nature·A ZychlinskyP J Sansonetti
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BernardiniP J Sansonetti
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Apr 1, 1985·Infection and Immunity·E V OaksS B Formal
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Jan 1, 1995·Methods in Cell Biology·A J McGahonD R Green
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Mar 22, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T HayashiH Shinagawa
Jun 22, 2001·The Journal of Biological Chemistry·T ToyotomeC Sasakawa
Aug 21, 2003·Microbial Pathogenesis·Nurmohammad ShaikhHaruo Watanabe
Dec 4, 2004·Science·Michinaga OgawaChihiro Sasakawa

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Citations

Dec 7, 2007·Nature Reviews. Microbiology·Michinaga OgawaChihiro Sasakawa
Sep 2, 2009·Journal of Molecular Cell Biology·Guy Tran Van Nhieu, Julie Guignot
Oct 16, 2007·Clinical Microbiology Reviews·Bryan CoburnB Brett Finlay
Sep 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Toru TobeMark J Pallen
Apr 19, 2011·Nihon saikingaku zasshi. Japanese journal of bacteriology·Haruo Watanabe
Nov 30, 2011·The Journal of Cell Biology·Anne Marie KrachlerKim Orth
Mar 26, 2016·Frontiers in Cellular and Infection Microbiology·Hervé Agaisse
May 30, 2015·Trends in Microbiology·Carole J KuehlHervé Agaisse
Jan 23, 2009·Current Opinion in Microbiology·Claude Parsot
May 12, 2011·Drug Discovery Today·Monireh Torabi-RahvarAmir Hassan Zarnani
Aug 18, 2004·Trends in Molecular Medicine·Tracy HussellAndrew E Williams
Apr 11, 2017·Frontiers in Cellular and Infection Microbiology·Emily Mattock, Ariel J Blocker
Feb 13, 2021·International Journal of Molecular Sciences·Korinn N Murphy, Amanda J Brinkworth
Apr 7, 2019·Microbiology Spectrum·Pamela Schnupf, Philippe J Sansonetti

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