OsPRA1 plays a significant role in targeting of OsRab7 into the tonoplast via the prevacuolar compartment during vacuolar trafficking in plant cells.

Planta
Jae Bok HeoJeong Dong Bahk

Abstract

In yeast and mammals, the Yip/PRA1 family of proteins has been reported to facilitate the delivery of Rab GTPases to the membrane by dissociating the Rab-GDI complex during vesicle trafficking. Recently, we identified OsPRA1, a plant Yip/PRA1 homolog, as an OsRab7-interacting protein that localizes to the prevacuolar compartment, which suggests that it plays a role in vacuolar trafficking of plant cells. Here, we show that OsPRA1 is essential for vacuolar trafficking and that it has molecular properties that are typical of the Yip/PRA1 family of proteins. A trafficking assay using Arabidopsis protoplasts showed that the point mutant OsPRA1((Y94A)) strongly inhibits the vacuolar trafficking of cargo proteins, but has no inhibitory effect on the plasma membrane trafficking of H(+)-ATPase-GFP, suggesting its specific involvement in vacuolar trafficking. Moreover, OsPRA1 was shown to be an integral membrane protein, suggesting that its two hydrophobic domains may mediate membrane integration, and its cytoplasmic N- and C-terminal regions were found to be important for binding to OsRab7. OsPRA1 also interacted with OsVamp3, implying its involvement in vesicle fusion. Finally, we used a yeast expression system to show that OsPRA1 opp...Continue Reading

References

Apr 12, 1996·Science·J E Rothman, F T Wieland
Dec 2, 1998·The Journal of Cell Biology·L Jiang, J C Rogers
Apr 7, 2000·The Journal of Biological Chemistry·D M HuttJ K Ngsee
Jul 11, 2002·The Journal of Biological Chemistry·Pierre-Yves GougeonJohnny K Ngsee
Mar 27, 2003·The Journal of Biological Chemistry·Jemima BarrowmanSusan Ferro-Novick
Oct 15, 2003·The Journal of Cell Biology·Matthew HeidtmanCharles Barlowe
Oct 24, 2003·Nature·Ulf SivarsSuzanne R Pfeffer
Jan 1, 2004·Plant & Cell Physiology·Min Yeop NahmJeong Dong Bahk
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Suzanne Pfeffer, Dikran Aivazian
Oct 19, 2005·Plant & Cell Physiology·Jae Bok HeoJeong Dong Bahk
Feb 14, 2006·Methods in Enzymology·Ulf SivarsSuzanne Pfeffer
Aug 17, 2006·Nature Reviews. Molecular Cell Biology·Reinhard Jahn, Richard H Scheller
May 17, 2007·Nature Reviews. Molecular Cell Biology·Martin Lowe, Francis A Barr
Jun 15, 2007·International Review of Cytology·Merran C Derby, Paul A Gleeson
May 9, 2008·European Journal of Pediatrics·Lucien Corbeel, Kathleen Freson
Jun 28, 2008·Plant Physiology·Claire Lessa Alvim KameiLieven De Veylder
Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·M Fukuda

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