OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice.

Chromosoma
Hengxiu YuZhukuan Cheng

Abstract

Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination. In the present study, we silenced the SPO11-1 gene in rice (Oryza sativa) using RNAi. Rice plants with loss-of-function of OsSPO11-1 have no apparent growth defects during vegetative development, but homologous chromosome pairing and recombination are significantly obstructed. Telomeres can be assembled as bouquet during the zygotene stage of the OsSPO11-1-deficient plants, just as that in wild type. Although the two axial-associated proteins, REC8 and PAIR2, are loaded onto the chromosomes, the depletion of PAIR2 from the chromosomes is much later than in wild type. The central element of the synaptonemal complex (SC), ZEP1, does not load onto the chromosomes normally, implying that SC formation is disturbed severely. The crossover protein, MER3, isn't efficiently assembled onto chromosomes and the lack of bivalent suggests that crossovers are also affected in the absence of OsSPO11-1. Thus, OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.

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Citations

Jun 21, 2011·Yi chuan = Hereditas·Jun ChenQiong Luo
Jul 28, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hua ZhangYang-Sheng Li
Mar 21, 2016·Seminars in Cell & Developmental Biology·Thomas RobertMathilde Grelon
Apr 18, 2012·The Plant Journal : for Cell and Molecular Biology·Jianhui JiZhukuan Cheng
Mar 25, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qiong LuoZhukuan Cheng
Dec 11, 2014·Annual Review of Plant Biology·Raphaël MercierMathilde Grelon
Feb 19, 2015·Frontiers in Plant Science·Zhigang WuQiong Luo
May 23, 2014·Frontiers in Plant Science·Ding TangZhukuan Cheng
Aug 12, 2014·Frontiers in Plant Science·Melania Collado-RomeroPilar Prieto
Sep 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jianhui JiZhukuan Cheng
Sep 24, 2015·PloS One·Bingwei ZhangHengxiu Yu
Jan 31, 2019·Journal of Integrative Plant Biology·En XieZhukuan Cheng
Apr 21, 2019·The Plant Journal : for Cell and Molecular Biology·Yang TaoShuangcheng Li
Jun 19, 2014·G3 : Genes - Genomes - Genetics·Han ZhangVirginia Walbot
Oct 7, 2019·Journal of Experimental Botany·Zhenyi ChangXiaoyan Tang
Jun 20, 2020·The Plant Journal : for Cell and Molecular Biology·Olivier Da InesPierre Sourdille
Jul 1, 2020·The Plant Journal : for Cell and Molecular Biology·Fatiha BenyahyaPierre Sourdille
Jul 26, 2019·Proceedings of the National Academy of Sciences of the United States of America·Zhihui XueZhukuan Cheng
Jun 15, 2019·Plant Biotechnology Journal·Ian FayosEmmanuel Guiderdoni
Aug 26, 2020·Journal of Experimental Botany·Ian FayosEmmanuel Guiderdoni
Jan 11, 2021·Current Opinion in Plant Biology·Imtiyaz Khanday, Venkatesan Sundaresan
Mar 26, 2021·Frontiers in Plant Science·Adil AbbasLiyong Cao
Aug 6, 2021·Frontiers in Plant Science·Pallas KuoChristophe Lambing
Aug 17, 2021·The Plant Journal : for Cell and Molecular Biology·Tingting ZhaoZhukuan Cheng

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