Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells

Oncotarget
Moses O OyewumiFayez F Safadi

Abstract

The potential application of GPNMB/OA as a therapeutic target for lung cancer will require a greater understanding of the impact of GPNMB/OA ectodomain (ECD) protein shedding into tumor tissues. Thus, in this work we characterized GPNMB/OA expression and extent of shedding of its ECD protein while evaluating the impact on lung cancer progression using three non-small cell lung cancer (NSCLC) cell lines: A549, SK-MES-1 and calu-6. We observed a direct correlation (R2 = 0.89) between GPNMB/OA expression on NSCLC cells and the extent of GPNMB/OA ECD protein shedding. Meanwhile, siRNA-mediated knockdown of GPNMB/OA in cancer cells significantly reduced GPNMB/OA ECD protein shedding, migration, invasion and adhesion to extracellular matrix materials. Also, exogenous treatment of cancer cells (expressing low GPNMB/OA) with recombinant GPNMB/OA protein (rOA) significantly facilitated cell invasion and migration, but the effects of rOA was negated by inclusion of a selective RGD peptide. Further studies in athymic (nu/nu) mice-bearing calu-6 showed that intratumoral supplementation with rOA effectively facilitated in vivo tumor growth as characterized by a high number of proliferating cells (Ki67 staining) coupled with a low number of ...Continue Reading

References

Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·T A SullivanP B Lindgren
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Aug 16, 2005·American Journal of Physiology. Cell Physiology·Takayuki OgawaNatsuo Yasui
Aug 19, 2005·American Journal of Respiratory Cell and Molecular Biology·York E Miller
Apr 13, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chien-Tsun KuanDarell D Bigner
May 9, 2006·Cancer Metastasis Reviews·Joaquín ArribasBelén Santiago-Josefat
Oct 13, 2006·Journal of Cellular Physiology·Samir M AbdelmagidFayez F Safadi
Oct 24, 2007·Molecular Cancer Research : MCR·April A N RosePeter M Siegel
Feb 22, 2008·Current Pharmaceutical Biotechnology·Marcia L MossPeter J Dempsey
Jun 17, 2008·Experimental Cell Research·Samir M AbdelmagidFayez F Safadi
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Etmar BulkCarsten Müller-Tidow
Feb 3, 2009·Journal of Neurochemistry·Edward Parkin, Benjamin Harris
Dec 24, 2009·Nature Reviews. Cancer·Jay S Desgrosellier, David A Cheresh
Mar 11, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·April A N RosePeter M Siegel
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Sep 13, 2011·Clinical Proteomics·Michael J DuffyPatricia M McGowan
Aug 17, 2012·The American Journal of Surgical Pathology·Satu Maria RemesJohanna Arola
May 10, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ying-Na LiWei-Lin Yang
Nov 23, 2013·Journal of Cellular Physiology·Gregory R SondagFayez F Safadi
Dec 12, 2013·American Journal of Physiology. Endocrinology and Metabolism·Xiao M PeiParco M Siu
Jan 15, 2014·Journal of Cellular Biochemistry·Fouad M MoussaFayez F Safadi
Feb 6, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Robert E MillerWilliam C Dougall
May 27, 2014·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Sekhar PalAmitava Chatterjee
Aug 2, 2014·Cell·Ruth Scherz-ShouvalSusan Lindquist
Sep 10, 2014·International Journal of Cell Biology·Moses O OyewumiFayez F Safadi

❮ Previous
Next ❯

Citations

Sep 1, 2017·Molecular Neurobiology·Kevin M BudgeFayez F Safadi
Apr 27, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yi-Xiang ZhangJiang-Gen Yang
Sep 10, 2019·Journal of Cellular Physiology·Feifei RenYang Mi
May 24, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pei-Suen Tsou, Amr H Sawalha
Mar 16, 2017·Journal of Cellular Physiology·Oneida A ArosarenaFayez F Safadi
Jun 1, 2018·Journal of Cellular and Molecular Medicine·Ran WeiHang Fai Kwok
Jun 21, 2019·Intractable & Rare Diseases Research·Jun-Ichi SatohYuko Saito
Dec 12, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jin-Sung ChungKiyoshi Ariizumi

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
xenografts
xenograft
Protein Assay
electrophoresis
PCR

Software Mentioned

CyQuant
GraphPad Prism

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.