Osteoarthritis in cynomolgus macaques. II. Detection of modulated proteoglycan epitopes in cartilage and synovial fluid

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
C S CarlsonB Caterson

Abstract

The purpose of the present study was to determine the usefulness of the monoclonal antibodies 7-D-4 and 3-B-3 as biomarkers of severity of naturally occurring osteoarthritis in the knee joints of adult cynomolgus macaques. The antibodies were used to immunolocate chondroitin sulfate proteoglycan epitopes in articular cartilage or synovial fluid from knee joints with a range in severity of osteoarthritis. The joints were examined radiographically, grossly, microradiographically, and histologically to characterize the severity of disease, and the results of three different methods of proteoglycan analysis (immunohistochemistry, enzyme-linked immunosorbent assay, and Western blot analysis) were compared. Subjectively, the degree of positive immunostaining for 7-D-4 was minimal in normal sites and increased as damage to articular cartilage increased. The scores for 7-D-4 immunostaining in the medial tibial plateau (the site most severely involved in this model) were correlated significantly with severity of damage to articular cartilage (p < 0.05, r2 = 0.50), thus supporting the subjective observations. The ratio of 7-D-4 to sulfated glycosaminoglycans in synovial fluid also was correlated with the score for 7-D-4 immunostaining in...Continue Reading

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Citations

Sep 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C S CarlsonC P Jerome
Feb 21, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Cathy S CarlsonVirginia B Kraus
Nov 1, 2013·ILAR Journal·Laura A CoxJohn L VandeBerg
May 22, 2010·The Veterinary Journal·Elaine R GarvicanJohn F Innes
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Mar 19, 2013·Osteoarthritis and Cartilage·T E MacriniL M Havill
Feb 10, 2017·Scientific Reports·Tarsis Ferreira GesteiraWinston W-Y Kao
Mar 29, 2006·The American Journal of Sports Medicine·Robert F LapradeCathy S Carlson
Jul 19, 2002·Arthritis and Rheumatism·Kimberley D HamCathy S Carlson
May 18, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·E LindhorstV B Kraus
Jan 24, 2012·International Journal of Experimental Pathology·Bruce Caterson
Sep 13, 2000·Journal of the American Geriatrics Society·S P MessierW H Ettinger
Oct 2, 1998·Clinical Orthopaedics and Related Research·G LovászJ V Luck
Aug 5, 2009·Biomarker Insights·Susan TsengPaul E Di Cesare
May 28, 2003·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Jun YeKenichi Shinomiya

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