Osteoarthritis in the temporo-mandibular joint (TMJ) of aged mice and the in vitro effect of TGF-beta 1 on cell proliferation, matrix synthesis, and alkaline phosphatase activity

Microscopy Research and Technique
E LivneIsrael Blumenfeld

Abstract

The temporo-mandibular joint of aged mice develops osteoarthritic (OA) degenerative lesions. Adult chondrocytes have a low rate of cell replication, and cartilage repair potential is very limited. One of the major problems in OA is the low rate of matrix synthesis and the inability of the chondrocytes to exceed the rate of matrix degradation. These combined factors lead to the overall destruction of the cartilage as seen in OA. Cartilage degradation is mediated by elevated proteolytic activity of enzymes. Among the enzymes degrading cartilage are the metalloproteinases, stromelysin and collagenase. Other proteinases that may potentially participate in matrix degradation are the lysosomal enzymes cathepsin B, D, and L, and acid phosphatase. On the other hand, alkaline phosphatase (ALP) is an enzyme that has been shown to be a marker for anabolic activity in skeletal tissues such as bone and cartilage. The cartilage of the mandibular condyle in the T-M-J from aged mice reveals OA lesions. An overall reduction of cell proliferation and sulfated proteoglycan synthesis has been also shown in this joint. In the present study the effects of hTGF-beta on the stimulation of DNA and sulfated GAG synthesis and ALP activity were studied. M...Continue Reading

References

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Sep 1, 1994·Osteoarthritis and Cartilage·E Livne

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Citations

Mar 28, 2006·Osteoarthritis and Cartilage·S OhnoW Knudson
May 6, 2004·Archives of Oral Biology·Michael S Detamore, Kyriacos A Athanasiou
Oct 15, 2003·Arthritis and Rheumatism·Maryse PicherGreta M Lee
Mar 13, 2009·Journal of Biomedical Materials Research. Part a·T KamiyaK Tanne
Feb 21, 2004·The Journal of Biological Chemistry·Ligong ChenAnne Woods
May 10, 2005·Archives of Oral Biology·M DelatteA M Kuijpers-Jagtman

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