Osteoblast biocompatibility of premineralized, hexamethylene-1,6-diaminocarboxysulfonate crosslinked chitosan fibers

Journal of Biomedical Materials Research. Part a
Marjorie A KiechelCaroline L Schauer

Abstract

Biopolymer-ceramic composites are thought to be particularly promising materials for bone tissue engineering as they more closely mimic natural bone. Here, we demonstrate the fabrication by electrospinning of fibrous chitosan-hydroxyapatite composite scaffolds with low (1 wt %) and high (10 wt %) mineral contents. Scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS) and unidirectional tensile testing were performed to determine fiber surface morphology, elemental composition, and tensile Young's modulus (E) and ultimate tensile strength (σUTS ), respectively. EDS scans of the scaffolds indicated that the fibers, crosslinked with either hexamethylene-1,6-diaminocarboxysulfonate (HDACS) or genipin, have a crystalline hydroxyapatite mineral content at 10 wt % additive. Moreover, FESEM micrographs showed that all electrospun fibers have diameters (122-249 nm), which fall within the range of those of fibrous collagen found in the extracellular matrix of bone. Young's modulus and ultimate tensile strength of the various crosslinked composite compositions were in the range of 116-329 MPa and 2-15 MPa, respectively. Osteocytes seeded onto the mineralized fibers were able to demonstrate good biocompatibility enhanc...Continue Reading

References

Feb 1, 1992·Calcified Tissue International·H C Margolis, E C Moreno
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Mar 21, 1998·Plastic and Reconstructive Surgery·A S BreitbartR T Grant
Aug 11, 1998·Journal of Biomedical Materials Research·A C WanG W Hastings
May 20, 1999·Journal of Biomedical Materials Research·C I ValloT R Cuadrado
Aug 14, 2001·Journal of Dental Research·Y YoshidaM Okazaki
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 12, 2002·Biomacromolecules·Eric R WelshRonald R Price
May 8, 2003·Journal of Biomedical Materials Research. Part a·Huang-Chien LiangHsing-Wen Sung
Jul 31, 2003·Journal of Biomedical Materials Research. Part a·Benjamin G KeselowskyAndrés J García
Sep 2, 2003·International Journal of Cancer. Journal International Du Cancer·Thomas D SchmittgenBrian A Zakrajsek
Mar 30, 2004·Biomaterials·C C RibeiroM A Barbosa
Apr 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Benjamin G KeselowskyAndrés J García
Sep 1, 2005·Journal of Biomaterials Science. Polymer Edition·Anuradha SubramanianHsin-Yi Lin
Apr 1, 2006·Journal of Nanoscience and Nanotechnology·Vinoy ThomasElijah Nyairo
Jun 30, 2007·Journal of Biomedical Materials Research. Part a·Sashka DimitrievskaL'Hocine Yahia
Aug 19, 2007·Biomacromolecules·Jessica D Schiffman, Caroline L Schauer
Sep 10, 2008·Acta Biomaterialia·Moncy V JoseElijah Nyairo
Jan 6, 2009·Acta Biomaterialia·W W Thein-Han, R D K Misra
May 6, 2011·JOM : the Journal of the Minerals, Metals & Materials Society·Nichols W MeghriUlrike G K Wegst
Aug 16, 2011·International Journal of Biological Macromolecules·Hilal Turkoglu Sasmazel
May 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Biman B MandalDavid L Kaplan
May 26, 2012·Journal of the Royal Society, Interface·Marjorie S AusteroCaroline L Schauer
Jan 26, 2013·Journal of the Royal Society, Interface·Amalie E DoniusUlrike G K Wegst
Jul 1, 2012·Carbohydrate Polymers·Riccardo A A MuzzarelliAntonio Gigante

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Citations

Aug 20, 2015·Marine Drugs·Randy Chi Fai CheungWai Yee Chan
Apr 24, 2016·International Journal of Biological Macromolecules·Riccardo A A MuzzarelliAntonio Gigante
Dec 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shan-Hui HsuCheng-Wei Chen
Feb 18, 2015·Journal of Biomedical Materials Research. Part a·Laura T BeringerCaroline L Schauer

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