Osteoblast-conditioned media influence the expression of E-selectin on bone-derived vascular endothelial cells

Journal of Cellular Biochemistry
Lauren A MakuchCarol V Gay

Abstract

Breast cancer cells frequently metastasize to the ends of long bones, ribs and vertebrae, structures which contain a rich microvasculature that is closely juxtaposed to metabolically active trabecular bone surfaces. This study focuses on the effects of osteoblast secretions on the surface presentation of adhesive proteins on skeletal vascular endothelial cells. Vascular endothelial cells were isolated from trabecular bone regions of the long bones of 7-week-old Swiss Webster mice and also from the central marrow cavity where trabecular bone is absent. Both types of endothelial cells were placed in culture for 7 days, then exposed 24 h to conditioned media from MC3T3-E1 osteoblasts. Conditioned medium (CM) from two different stages of osteoblast development were tested: (1) from immature MC3T3-E1 cells cultured for 5-7 days and (2) from mature MC3T3-E1 cells cultured for 28-30 days. The immature osteoblasts were in a stage of rapid proliferation; the mature osteoblasts formed a matrix that mineralized. Following exposure to the conditioned media, the vascular cells were exposed to anti-P-selectin, anti-E-selectin, anti-ICAM-1, and anti-VCAM-1 to detect the corresponding adhesive proteins on their surfaces. Breast cancer cells ar...Continue Reading

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Citations

Dec 12, 2007·Cancer Metastasis Reviews·Karen M BussardAndrea M Mastro
Sep 13, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaojia ChenQiuling Xie
Mar 21, 2007·Journal of Cellular Biochemistry·Donna M Sosnoski, Carol V Gay

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