Osteoblast response to the elastic strain of metallic support

Journal of Biomechanics
M Lewandowska-SzumiełW Marczyński

Abstract

It is known that metallic elements of joint endoprostheses undergo elastic strain due to their mechanical function. This is one of the factors which may be responsible for the loosening of endoprostheses. Since mechanisms involved in it remain unclear, it seems valuable to verify if cells responsible for bone regeneration are affected by a strain of the implant. Our experiment examines the influence of elastic strain applied to Ti6Al4V samples on osteoblasts cultured on their surface in vitro. Human bone-derived cells are observed in contact with metallic plates. Titanium alloy was chosen as a support since it is one of the most commonly used materials for stems in joint endoprostheses. Cyclic elastic deformation of 0.1% was applied to the support once daily for 7 days. Two thousand cycles were applied each time. Samples which were not subject to strain served as control. After the observation period XTT assay was performed, alkaline phosphatase activity as well as osteocalcin concentration and nitric oxide secretion were determined and compared with the results obtained in the control group. It was found that the number of viable cells in the mechanically stimulated population was significantly higher than in control, while bo...Continue Reading

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Citations

Oct 1, 2013·Journal of Materials Science. Materials in Medicine·Malgorzata Lewandowska-Szumiel, Ilona Kalaszczynska
Jun 16, 2012·Journal of Biomedicine & Biotechnology·Joana ReisJosé Potes
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·Laura SaldañaNuria Vilaboa
Jun 15, 2010·Journal of Biomechanics·J A Sanz-HerreraJ M García-Aznar
Feb 2, 2010·Journal of Biomechanics·C FriasA T Marques
Sep 22, 2009·Journal of Dental Research·N SatoT Ogawa
May 28, 2015·Journal of Biomedical Materials Research. Part a·Anna Scislowska-CzarneckaElzbieta Kolaczkowska

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