Osteoblastic cells have L-type calcium channels.

Bone and Mineral
C GrygorczykJ Ferrier

Abstract

Whole cell patch clamp studies on osteoblast-like rat osteosarcoma cells (ROS 17/2.8) show the existence of L-type calcium channels in the cell membrane. Measurements were carried out at both 21 and 37 degrees C. With isotonic CsCl in the pipette and a bathing medium containing either 110 or 10 mM Ba2+, a strong depolarizing pulse was required to activate an inward current. The current-voltage relationship (I-V) of this inward current showed a maximum amplitude near +30 mV at 21 and 37 degrees C, with 110 mM Ba2+ in the bathing medium, and near +10 mV at 37 degrees C with 10 mM Ba2+. At both 21 and 37 degrees C the dihydropyridine, BAY K 8644 (2 microM), increased this current and shifted the I-V maximum to less positive potentials, while nifedipine (5 microM) reduced the current. Cd2+ (50 microM) and Co2+ (100 microM) blocked the current. At 21 degrees C the measured inward current showed a slow inactivation, with a time constant of some hundreds of milliseconds. At 37 degrees C, inactivation was considerably faster. The current was suppressed by holding the membrane potential more positive than -30 mV. These data are strong evidence that ROS 17/2.8 cells have a significant number of 'L-type' calcium channels.

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Citations

Dec 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J H RaveslootP J Nijweide
Dec 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D L YpeyP J Nijweide
Sep 1, 1991·The Journal of Membrane Biology·J FerrierS L Xia
Jun 13, 2001·Biochemical and Biophysical Research Communications·Y GuS J Publicover
Oct 3, 1999·Medical & Biological Engineering & Computing·A J el HajS J Publicover
Dec 1, 1992·Matrix : Collagen and Related Research·G P Vadiakas, A J Banes
Jan 6, 2010·Journal of Cellular Physiology·Henrique B SilvaJosé H M Nascimento
Oct 1, 1991·Bone and Mineral·C Richter, J Ferrier
Mar 11, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·M A WoodA J El Haj
Oct 18, 2005·Journal of Cellular Biochemistry·Majd Zayzafoon
Mar 16, 2021·Current Osteoporosis Reports·Christian S WrightWilliam R Thompson

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