Osteoblasts respond to hydroxyapatite surfaces with immediate changes in gene expression

Journal of Biomedical Materials Research. Part a
Jianwei XieLaura R McCabe

Abstract

Bone mineral contains hydroxyapatite (HA). This is the surface that mature osteoblasts and osteocytes interact with. Synthetic HA is widely used in orthopedic surgeries as an implant or implant coating. The bone-like HA surfaces increase implant union and bone formation; however, the mechanisms accounting for this effect on osteoblasts are not known. In this study, we compared gene expression profiles of osteoblasts responding to HA or plastic surfaces for 24 h. Expression profiles were also compared between HA discs processed with gravity-sieved compared with combined gravity and air-jet-sieved HA powders. The latter, composed of smaller HA particles, exhibits an increase in grain boundary surface area. Discs made with either HA powder similarly up-regulated osteoblast expression of 10 genes (including proliferin 3, Glvr-1, DMP-1, and tenascin C) and down-regulated 15 genes (such as osteoglycin) by more than 2-fold compared with plastic surfaces. The overall changes are indicative of an immediate (24-h) response to the HA surface and a trend toward osteoblast differentiation. In addition, subsets of modulated genes exist that are unique to each HA subtype. Taken together, we identified HA responsive genes evident within 24 h o...Continue Reading

References

Jan 1, 1992·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J B Lian, G S Stein
Jan 1, 1995·The Journal of Bone and Joint Surgery. American Volume·J E DaltonJ F Kay
Jun 1, 1994·Journal of Nutritional Science and Vitaminology·Y ToriiN Tsukagoshi
Jan 1, 1996·Connective Tissue Research·M MacDougallD Simmons
Jan 24, 1998·Journal of Cellular Biochemistry·H A FeisterJ P Bidwell
Apr 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M MacDougallJ Chen
May 23, 1998·Genomics·T VaccariM E Bianchi
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A JikkoC H Damsky
Sep 29, 2000·Circulation Research·S JonoC M Giachelli
Mar 13, 2001·Biochemical and Biophysical Research Communications·W HuangT A Miller
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A NifujiM Noda
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·D J ToftD I Linzer
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jian Q FenMary MacDougall
May 8, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jelica Gluhak-HeinrichDubravko Pavlin
Jun 17, 2003·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·F CarinciA Piattelli
Aug 21, 2003·Lasers in Medical Science·S HamajimaY Abiko
Aug 27, 2003·Journal of the American Society of Nephrology : JASN·Cecilia M Giachelli
Nov 19, 2003·Journal of Biomedical Materials Research. Part a·R ShuL R McCabe

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Citations

Oct 25, 2007·Journal of Materials Science. Materials in Medicine·J VenugopalS Ramakrishna
Jun 28, 2011·Journal of Materials Science. Materials in Medicine·K HristovaG Altankov
Jan 27, 2010·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Otilia ZarnescuLucia Moldovan
May 25, 2012·Chemical Reviews·Akon HiguchiAkihiro Umezawa
Jul 17, 2012·Photomedicine and Laser Surgery·Nai-Chia TengHaw-Ming Huang
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Swaminathan SethuramanCato T Laurencin
Aug 29, 2007·International Journal of Nanomedicine·Ian O SmithMelissa J Baumann
Feb 9, 2012·International Journal of Molecular Sciences·Chiara GardinBarbara Zavan
May 29, 2014·Journal of Materials Science. Materials in Medicine·V CampanaG Logroscino
Aug 12, 2015·Journal of Functional Biomaterials·Sergey V Dorozhkin
Jul 8, 2014·International Journal of Molecular Sciences·Bill G X ZhangPeter F M Choong
Mar 17, 2011·Advances in Chronic Kidney Disease·Clemens Bergwitz, Harald Jüppner
Apr 24, 2008·Journal of Biomedical Materials Research. Part a·Liwen LinYang Leng
May 4, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Maiko HagaTakeyasu Maeda
Feb 15, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hassan BabikerSøren Overgaard
Feb 2, 2011·Journal of Biomedical Materials Research. Part a·Adil AkkouchMahmoud Rouabhia
Oct 12, 2013·Journal of Biomedical Materials Research. Part a·Xiaoying LüLifeng Zhao
Sep 27, 2014·Journal of Biomedical Materials Research. Part a·AniketAhmed El-Ghannam
Dec 6, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Erica G TierneyFergal J O'Brien
Dec 24, 2010·Colloids and Surfaces. B, Biointerfaces·Yoon Jung HongWoo-Kul Lee
Aug 19, 2016·ACS Applied Materials & Interfaces·Gráinne M CunniffeFergal J O'Brien
Apr 4, 2013·Technology in Cancer Research & Treatment·Ecaterina AndronescuAnisoara Cimpean
May 4, 2018·Journal of Biomaterials Applications·Kanwal IlyasAbdul S Khan
Jun 10, 2009·Tissue Engineering. Part C, Methods·Michael KeeneyAbhay Pandit
Feb 12, 2009·Tissue Engineering. Part B, Reviews·Michael Keeney, Abhay Pandit
Apr 15, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·I O SmithE D Case
Sep 2, 2006·Journal of Biomedical Materials Research. Part a·Kirstin SuckCornelia Kasper
Feb 17, 2005·Journal of Biomedical Materials Research. Part a·Jianwei XieLaura R McCabe
Aug 19, 2007·Journal of Biomedical Materials Research. Part a·J VenugopalS Ramakrishna
Apr 26, 2008·Journal of Biomedical Materials Research. Part a·E A dos SantosK Anselme

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