Osteocalcin production in primary osteoblast cultures derived from normal and Hyp mice

Endocrinology
T O CarpenterC M Gundberg

Abstract

Rickets and osteomalacia are characteristic features of the Hyp mouse model of human X-linked hypophosphatemia. Hyp mice demonstrate elevated circulating osteocalcin levels, as well as altered regulation of osteocalcin by 1,25(OH)2D3. Whether this osteocalcin abnormality is intrinsic to the osteoblast, or mediated by the in vivo milieu, has not been established. We therefore characterized osteocalcin production and its regulation by 1,25(OH)2D3 in primary cultures of murine osteoblasts and examined osteocalcin and its messenger RNA in response to 1,25(OH)2D3 in cultures of Hyp mouse-derived osteoblasts. Cell viability and osteocalcin production are optimal when murine cells are harvested within 36 h of age. Murine primary osteoblast cultures mineralize and produce osteocalcin in a maturation-dependent fashion (as demonstrated in other species), and continuous exposure to 1,25(OH)2D3, beginning at day 9 of culture, inhibits osteoblast differentiation and osteocalcin production and prevents mineralization of the culture. However, in contrast to other species, exposure to 1,25(OH)2D3, added later (days 17-25) in culture, does not stimulate osteocalcin but arrests osteocalcin production at current levels. Ambient media levels of os...Continue Reading

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Citations

May 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Thomas L McCarthyMichael Centrella
Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Peter S N Rowe
Jan 23, 2010·Molecular Endocrinology·Thomas L McCarthy, Michael Centrella
Oct 9, 2014·Journal of Cellular Physiology·Thomas L McCarthy, Michael Centrella
Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A F RuchonG Boileau
Aug 29, 2013·Journal of Biomedical Materials Research. Part a·M S HasanC A Scotchford
Oct 11, 2016·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Philipp KauffmannRudolf Matthias Gruber
Jun 26, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoko UchidaManabu Morita
Jul 18, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Meerim ParkYoung Ho Lee
Oct 1, 1998·The American Journal of Physiology·Z S XiaoL D Quarles
Oct 2, 2007·The Journal of Biological Chemistry·Thomas L McCarthyMichael Centrella
Sep 3, 2003·The Journal of Biological Chemistry·Thomas L McCarthyMichael Centrella
Aug 10, 2004·The Journal of Biological Chemistry·Weizhong ChangThomas L McCarthy

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