Osteoclastogenesis in peripheral blood mononuclear cell cultures of periprosthetic osteolysis patients and the phenotype of T cells localized in periprosthetic tissues

Biomaterials
Ilaria RoatoRiccardo Ferracini

Abstract

Arthroplasty is a very successful medical procedure. Failures depend on aseptic loosening caused by periprosthetic osteolysis, where T cells have a contradictory role. We analyzed osteoclastogenesis in peripheral blood mononuclear cell (PBMC) cultures of periprosthetic osteolysis patients and the phenotype of T cells localized in periprosthetic tissues. We enrolled 45 subjects with periprosthetic osteolysis (15), stable prosthesis (15) and healthy controls (15). We performed PBMC cultures to study osteoclastogenesis. Osteoclasts and T cell phenotype were examined by immunohistochemistry, immunofluorescence and flow citometry. Periprosthetic osteolysis patients showed spontaneous osteoclastogenesis, which was inhibited by RANK-Fc and T cell depletion. In periprosthetic osteolysis patients' PBMC cultures, CD4 and CD8 T cells increased and CD8 T cells did not express CD25. In periprosthetic tissues T cells were close to osteoclasts, suggesting their interaction. Local CD8 T cells showed a regulatory phenotype, expressing CD25 and FoxP3, while CD4 T cells did not express activation markers. Our data suggest that, in an early stage of periprosthetic osteolysis, T cells may promote osteoclastogenesis, whereas subsequently osteoclasts...Continue Reading

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Citations

Jan 1, 2012·BoneKEy Reports·Lucia D'Amico, Ilaria Roato
Mar 29, 2014·Clinical Orthopaedics and Related Research·D Rick SumnerEd Purdue
Sep 4, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nadim J HallabJoshua J Jacobs
Sep 6, 2013·Immunological Investigations·Patrizia D'Amelio
May 23, 2015·Journal of Immunology Research·Giorgio MoriGiacomina Brunetti
May 20, 2016·BoneKEy Reports·Patrizia D'Amelio, Francesca Sassi
Oct 1, 2011·International Journal of Immunopathology and Pharmacology·E CenniN Baldini
Dec 8, 2010·Clinical Orthopaedics and Related Research·Ryan M BaxterMarla J Steinbeck
Aug 13, 2020·Molecular Genetics and Metabolism·P A RozenfeldM V Delpino
Jun 5, 2021·Acta Biomaterialia·Gemma MestresAnna Diez-Escudero

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