Osteoclasts are not activated in middle ear cholesteatoma

Journal of Bone and Mineral Metabolism
Hiroki KoizumiAkinori Sakai

Abstract

It is unclear whether osteoclasts are present and activated in cholesteatomas. We explored the expression of messenger RNA (mRNA) for osteoclast biomarkers and regulating factors in middle ear cholesteatomas to elucidate the level of osteoclast activity in this disease. Bone powder was collected from 14 patients with cholesteatomatous and noncholesteatomatous chronic otitis media during tympanomastoidectomy, separately from cortical bone of the mastoid (clean bone powder), from bone neighboring cholesteatoma (cholesteatomatous bone powder), and from bone of the air cells and antrum of noncholesteatomatous chronic otitis media patients (noncholesteatomatous bone powder). The samples collected were soaked in TRIzol reagent, and total RNA was extracted and purified by the acid guanidinium thiocyanate-phenol-chloroform method, followed by the use of magnetic bead technology. The sample was then subjected to quantitative reverse transcription polymerase chain reaction for receptor activator of nuclear factor κB (RANK), tartrate-resistant acid phosphatase (TRAP), cathepsin K (CTSK), osteoclast-associated receptor (OSCAR), calcitonin receptor (CALCR), matrix metalloproteinase 9 (MMP9), receptor activator of nuclear factor κB ligand (R...Continue Reading

References

Nov 1, 1988·The Annals of Otology, Rhinology, and Laryngology·R A Chole
Oct 1, 1974·The Journal of Laryngology and Otology·J ThemsenH K Kristensen
Nov 1, 1969·The Laryngoscope·G Schechter
Feb 1, 1972·The Annals of Otology, Rhinology, and Laryngology·D J Lim, W H Saunders
Jul 1, 1982·American Journal of Otolaryngology·B J GantzU Fisch
Jun 1, 1981·Clinical Otolaryngology and Allied Sciences·J ThomsenM Balslev Jøorgensen
Jan 1, 1980·Archives of Oto-rhino-laryngology·K TanakaT Hirai
Jun 1, 1995·The Annals of Otology, Rhinology, and Laryngology·Y Uno, R Saito
May 15, 2001·Acta Oto-laryngologica·T H YoonH J Kim
Jan 24, 2002·The Journal of Experimental Medicine·Nacksung KimYongwon Choi
Mar 5, 2003·The Laryngoscope·Michael HamzeiHolger Sudhoff
Aug 1, 1958·The Journal of Laryngology and Otology·J F BIRRELL
Dec 1, 1951·The Annals of Otology, Rhinology, and Laryngology·T E WALSHJ H OGURA
May 10, 2005·Biochimica Et Biophysica Acta·Julia S KuliwabaDavid M Findlay
Jul 11, 2006·The Laryngoscope·Jin-Hyeok JeongYong-Wook Park
Apr 9, 2008·Archives of Biochemistry and Biophysics·Brendan F Boyce, Lianping Xing
Sep 10, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Makoto MiyamotoRyoetsu Imai
Dec 18, 2009·American Journal of Otolaryngology·Jerzy KuczkowskiEwa Iżycka-Świeszewska
Jul 27, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sara M Mantila RoosaCharles H Turner
Dec 22, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katharina NemethClaudia Goettsch
Aug 16, 2011·The Journal of Clinical Investigation·Alexander David BarrowJohn Trowsdale
Jan 21, 2012·BMC Biotechnology·Lauren E CarterZ Elizabeth Floyd
Oct 15, 2013·The Laryngoscope·Khac-Hung NguyenYoshiaki Doi
Oct 30, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carlo PalmieriJanet Brown
Mar 19, 2014·The Annals of Otology, Rhinology, and Laryngology·Hideaki SuzukiKoichi Hashida
Mar 26, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·A-L ThorsteinssonP Eiken

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Citations

Jul 9, 2016·Clinical Otolaryngology : Official Journal of ENT-UK ; Official Journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·H KoizumiH Suzuki
Mar 15, 2016·The Veterinary Clinics of North America. Small Animal Practice·Marije Risselada
Aug 31, 2016·Acta Oto-laryngologica·Hiroki KoizumiTakuro Kitamura
Apr 5, 2017·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Cuneyt M AlperDennis S Poe
Mar 5, 2019·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Serhan KeskinBurçin Erkal
Jun 30, 2019·Journal of the Association for Research in Otolaryngology : JARO·Ryusuke ImaiHidenori Inohara
Aug 6, 2021·The Journal of Laryngology and Otology·A ÖzgürM Çelebi

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