Osteocyte Characterization on Polydimethylsiloxane Substrates for Microsystems Applications

Journal of Biomimetics, Biomaterials, and Tissue Engineering
S L YorkMarnie M Saunders

Abstract

In the body, osteocytes reside in lacunae, lenticular shaped cavities within mineralized bone. These cells are linked to each other and surface-residing osteoblasts via physical channels known as gap junctions. It has been suggested that osteocytes sense mechanical load applied to bone and relay that signal to osteoclasts and osteoblasts. Current in vitro and in vivo models of mechanotransduction face temporal and spatial barriers. Recent advances in polydimethylsiloxane (PDMS) based microfabrication techniques may be able to overcome some of these hurdles. However, before the bone research field can effectively utilize microsystems techniques, fundamental groundwork must be completed. This study characterized the behaviour of osteocytes on PDMS coated with collagen type I (CTI) and provides the framework for bone cell mechanotransduction studies using microsystems. The goal was to determine whether osteocytes were adversely affected by the substrate material by comparing their behaviour to a standard glass substrate. In addition, optimal culture conditions and time points for growing osteocytes on PDMS substrates were determined. Results of this study suggested that use of PDMS does not adversely affect osteocyte behaviour. Fu...Continue Reading

References

Jan 14, 1986·Biochemical and Biophysical Research Communications·J S DavidsonE H Harley
Jan 8, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y KatoL F Bonewald
Nov 8, 2001·American Journal of Physiology. Cell Physiology·M M SaundersC R Jacobs
Jan 5, 2002·Analytical Chemistry·J C McDonaldG M Whitesides
Mar 22, 2005·The Journal of Biological Chemistry·Xiaofeng LiDianqing Wu
May 5, 2005·Cytokine & Growth Factor Reviews·Rutger L van BezooijenClemens W G M Löwik
Dec 20, 2007·The Journal of Biological Chemistry·Alexander G RoblingCharles H Turner
Mar 20, 2008·The Journal of Bone and Joint Surgery. American Volume·Peter ten DijkeRutger L van Bezooijen
Aug 12, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Amy L Paguirigan, David J Beebe
Apr 9, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ana SantosJenneke Klein-Nulend
May 8, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chuwen LinLin He
Mar 5, 2010·Journal of Dental Research·C GalliG M Macaluso
Jun 26, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Maret BauerAndreas Friedl
Dec 2, 2011·Methods in Molecular Biology·Jennifer Rosser, Lynda F Bonewald

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Citations

Mar 3, 2018·Experimental Cell Research·Estee L GeorgeMarnie M Saunders

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