Osteocyte-derived exosomes induced by mechanical strain promote human periodontal ligament stem cell proliferation and osteogenic differentiation via the miR-181b-5p/PTEN/AKT signaling pathway.

Stem Cell Research & Therapy
Pei-Ying LvYonglan Wang

Abstract

The oral cavity is a complex environment in which periodontal tissue is constantly stimulated by external microorganisms and mechanical forces. Proper mechanical force helps maintain periodontal tissue homeostasis, and improper inflammatory response can break the balance. Periodontal ligament (PDL) cells play crucial roles in responding to these challenges and maintaining the homeostasis of periodontal tissue. However, the mechanisms underlying PDL cell property changes induced by inflammatory and mechanical force microenvironments are still unclear. Recent studies have shown that exosomes function as a means of cell-cell and cell-matrix communication in biological processes. Human periodontal ligament stem cells (HPDLSCs) were tested by the CCK8 assay, EdU, alizarin red, and ALP staining to evaluate the functions of exosomes induced by a mechanical strain. MicroRNA sequencing was used to find the discrepancy miRNA in exosomes. In addition, real-time PCR, FISH, luciferase reporter assay, and western blotting assay were used to investigate the mechanism of miR-181b-5p regulating proliferation and osteogenic differentiation through the PTEN/AKT pathway. In this study, the exosomes secreted by MLO-Y4 cells exposed to mechanical st...Continue Reading

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Citations

Nov 22, 2020·Bone·Austin P Hensley, Audrey McAlinden
Mar 3, 2021·Archives of Oral Biology·Jeeranan ManokawinchokeHiroshi Egusa
May 10, 2021·Bone·Lilian I Plotkin, Joseph M Wallace

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Methods Mentioned

BETA
flow cytometry
transmission electron microscopy
Transfection
Assay
pull-down
PCR
Protein Assay
Fluorescence

Software Mentioned

PITA
ImageJ
SPSS
Exosome
miRanda
MS
microT
TargetScan

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