Osteocyte recruitment declines as the osteon fills in: interacting effects of osteocytic sclerostin and previous hip fracture on the size of cortical canals in the femoral neck

Bone
Jon PowerJ Reeve

Abstract

There is little information on the distribution of osteocytes within the individual cortical osteon, but using direct 3-D imaging in a single subject, Hannah et al. found a gradient with a two-fold higher density of cells adjacent to the cement line compared to near the canal. Since a limiting factor for bone formation might be the availability of osteoblasts due to their recruitment as osteocytes, we studied distributions of osteonal osteocytes in frozen sections of the femoral neck cortex. Osteocytes were stained with an anti-sclerostin antibody and counter-stained with toluidine blue. Adjacent sections were stained for alkaline phosphatase (ALP). Each osteonal osteocyte was categorised as being sclerostin-positive (scl+) or negative (scl-). ImageJ was used to measure the perimeter and area of each osteon and canal, while special purpose routines were used to measure the minimum distances of each osteocyte from the cement line and the canal. Canal area was strongly correlated with osteon area. Osteocytes were most dense close to the cement line; and their areal density within the matrix declined up to three-fold between the cement line and the canal, depending on osteon diameter. Large and small osteons had similar densities ...Continue Reading

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Citations

May 2, 2013·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·A BernhardB Busse
Nov 21, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Pawel SzulcRoland Chapurlat
Aug 7, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Samuel Gourion-ArsiquaudAdele L Boskey
May 2, 2013·American Journal of Physical Anthropology·John G SkedrosScott M Sorenson
Jan 11, 2019·American Journal of Physical Anthropology·Amy C BereshiemAmanda Alblas
Mar 10, 2017·BoneKEy Reports·Jonathan Reeve
Jan 26, 2021·Tissue Engineering. Part B, Reviews·Bei Chang, Xiaohua Liu

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