Osteodentin formation in rat incisor as visualized by radioautography after 3H-proline administration

The Anatomical Record
A C Karim, S P Pylypas

Abstract

Osteodentin formation was studied in rat incisor pulp after adriamycin administration. Male Sprague Dawley rats (100 +/- 5 gm) were injected intravenously with adriamycin (5 mg/kg body weight), and after 7 days they were again injected intravenously with 3H-proline (3 microCi/gm). These animals were killed in groups of three from 5 minutes to 4 hours after proline injection by perfusion with 3% phosphate-buffered formaldehyde followed by 2.5% phosphate-buffered glutaraldehyde. Control animals injected with only physiological saline, and 7 days later with 3H-proline (3 microCi/gm), and were killed at the same time intervals. Radioautography on sections showing osteodentin formation revealed that at 5 minutes after 3H-proline injection the labeling was located over the cells associated with the osteodentin matrix. At 1 hour after injection the labeling was located over the cells and the matrix, while at 4 hours the labeling was seen only over the matrix. It therefore appears that at least a proline-containing component of the osteodentin matrix is synthesized and secreted by the cells associated with it.

References

Oct 3, 1973·Histochemie. Histochemistry. Histochimie·B M Kopriwa
Mar 1, 1966·Journal of Dental Research·S Bernick
Sep 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Warshawsky, G Moore

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Citations

Dec 3, 2005·Cell Biology and Toxicology·T E JonesR B Johnson
May 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·C PhilippartN Dourov
Aug 1, 1991·Endodontics & Dental Traumatology·P J van Mullem

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