Abstract
It has been shown in experimental animals that the living cells in a bone autograft can make an important contribution to osteogenesis. However, some common clinical techniques, such as the topical use of antibiotic powders on grafts or on the graft bed, are likely to damage or kill the cells. In this experimental study in rats, bone isografts dusted with chloramphenicol or methicillin powder or with Polybactrin spray before subcutaneous implantation produced little or no new bone over a period of two weeks whereas untreated, control grafts showed abundant osteogenesis, as did grafts pretreated with solutions of antibiotics. The effect of short-term storage of the grafts for 3 to 24 hours in air, saline or culture medium before implantation was also examined. Grafts stored in culture medium generally did as well as, or better than, fresh control grafts whereas immersion in saline inhibited osteogenesis. The importance of these results for clinical bone grafting is discussed.
Citations
May 8, 2007·Clinical Orthopaedics and Related Research·Martin ButtaroFrancisco Piccaluga
Oct 17, 2019·Journal of Tissue Engineering and Regenerative Medicine·Qiang SunJill A Helms
Mar 1, 1986·Head & Neck Surgery·D S Soutar, W P Widdowson
Apr 5, 2008·Archives of Orthopaedic and Trauma Surgery·Uwe MausChristopher Niedhart
Mar 4, 2000·The Journal of Trauma·Y S ChanC H Shin
Sep 10, 2013·Implant Dentistry·Taketo KogaKazuyuki Ishihara
Oct 1, 2005·Acta Orthopaedica·Eivind WitsøKåre Bergh
Mar 4, 2011·The Journal of Bone and Joint Surgery. American Volume·Jennifer BauerAllison Gilmore
Nov 1, 1996·The Journal of Small Animal Practice·J A Culvenor, R J Parker
Nov 19, 2003·Acta Orthopaedica Scandinavica·Martin A ButtaroFrancisco Piccaluga
Jan 29, 2013·International Orthopaedics·Hans-Jürgen KockWerner Fabry
Oct 23, 1998·The Journal of Trauma·Y S ChanC H Shin
Jun 1, 1992·International Journal of Oral and Maxillofacial Surgery·M HsuK Nishijima
Jan 1, 1988·The Journal of Arthroplasty·J G Pedersen, B Lund
Oct 1, 1988·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·M Steiner, W K Ramp
Sep 1, 1996·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·R W MendicinoP Shromoff