Osteogenesis on nanoparticulate mineralized collagen scaffolds via autogenous activation of the canonical BMP receptor signaling pathway

Biomaterials
Xiaoyan RenJustine C Lee

Abstract

Skeletal regenerative medicine frequently incorporates deliverable growth factors to stimulate osteogenesis. However, the cost and side effects secondary to supraphysiologic dosages of growth factors warrant investigation of alternative methods of stimulating osteogenesis for clinical utilization. In this work, we describe growth factor independent osteogenic induction of human mesenchymal stem cells (hMSCs) on a novel nanoparticulate mineralized collagen glycosaminoglycan scaffold (MC-GAG). hMSCs demonstrated elevated osteogenic gene expression and mineralization on MC-GAG with minimal to no effect upon addition of BMP-2 when compared to non-mineralized scaffolds (Col-GAG). To investigate the intracellular pathways responsible for the increase in osteogenesis, we examined the canonical and non-canonical pathways downstream from BMP receptor activation. Constitutive Smad1/5 phosphorylation with nuclear translocation occurred on MC-GAG independent of BMP-2, whereas Smad1/5 phosphorylation depended on BMP-2 stimulation on Col-GAG. When non-canonical BMPR signaling molecules were examined, ERK1/2 phosphorylation was found to be decreased in MC-GAG but elevated in Col-GAG. No differences in Smad2/3 or p38 activation were detected. ...Continue Reading

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Citations

Dec 29, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·L CioccaR Scotti
Apr 23, 2016·Journal of the Mechanical Behavior of Biomedical Materials·D W WeisgerberB A C Harley
Aug 19, 2016·ACS Applied Materials & Interfaces·Gráinne M CunniffeFergal J O'Brien
Jun 7, 2017·Advanced Healthcare Materials·Justine C Lee, Elizabeth J Volpicelli
Dec 22, 2017·Tissue Engineering. Part a·Daniel W WeisgerberBrendan A C Harley
Feb 26, 2019·Journal of Tissue Engineering and Regenerative Medicine·Xiaoyan RenJustine C Lee
Jul 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wen SuXudong Li
Apr 7, 2019·Journal of the Mechanical Behavior of Biomedical Materials·Marley J DeweyBrendan A C Harley
May 24, 2019·Acta Biomaterialia·Aleczandria S TiffanyBrendan A C Harley
Jan 19, 2021·ACS Biomaterials Science & Engineering·Honglei WangChangsheng Liu
Sep 30, 2021·Advanced Healthcare Materials·Qi ZhouJustine C Lee

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