Osteogenic activity of MG63 cells on bone-like hydroxyapatite/collagen nanocomposite sponges.

Journal of Materials Science. Materials in Medicine
Teruaki YoshidaKazuo Takakuda

Abstract

The hydroxyapatite/collagen (HAp/Col) sponge with 95% (v/v) porosity was prepared by freeze-drying of a HAp/Col fiber suspension. MG63 cells were seeded onto the HAp/Col sponge and cultured under a pressure/perfusion condition with osteogenic supplements. A collagen (Col) sponge was used as a control. The cells with sponge were examined by a histology, total DNA content and gene expression. The cells showed good attachment and proliferation everywhere in the HAp/Col sponge, while the cells mainly proliferated at the peripheral part of the Col sponge. Thus, total DNA content in the HAp/Col sponges reached 1.8 times greater than that in the Col sponges at Day 21. Further, the cells and extracellular matrix only in the HAp/Col sponge were calcified, although the cells in both sponge evenly expressed osteogenic gene. These results suggest that the HAp/Col sponge could be useful as a scaffold for bone tissue engineering.

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Citations

Nov 6, 2013·Biological & Pharmaceutical Bulletin·Masanori Kikuchi
Mar 1, 2011·Acta Biomaterialia·Rene Olivares-NavarreteZvi Schwartz
Apr 5, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Masayoshi UezonoKeiji Moriyama
Sep 26, 2012·Journal of Biomedical Materials Research. Part a·Sandra C RodriguesFernando J Monteiro
Apr 22, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Despoina BrasinikaYiannis F Missirlis
Mar 20, 2012·The Spine Journal : Official Journal of the North American Spine Society·Rene Olivares-NavarreteBarbara D Boyan
Oct 14, 2017·Journal of Materials Science. Materials in Medicine·Naga Vijaya Lakshmi ManchinasettyMasanori Kikuchi
Dec 18, 2013·Advanced Healthcare Materials·Jianhua LiHong Liu
Oct 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bo LeiSu-Kyoung Chae
Apr 1, 2021·Journal of Biomedical Materials Research. Part a·Kaori Iwanami-KadowakiKeiji Moriyama

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