Osteogenic differentiation and calcification of human aortic smooth muscle cells is induced by the RCN2/STAT3/miR-155-5p feedback loop.

Vascular Pharmacology
Jian ZhaoZhihui Chang

Abstract

Vascular calcification (VC) is associated with the high morbidity and mortality of cardiovascular diseases in dialysis patients and is a process in which vascular smooth muscle cells (VSMCs) actively differentiate into osteoblast-like cells. Reticulocalbin-2 (RCN2) is involved in the process of osteogenic differentiation under diabetic conditions, but its regulatory role under hyperphosphatemic conditions and the related mechanisms remain unclear. In this study, the importance of the interactions among RCN2, STAT3 and miR-155-5p during the osteogenic differentiation and calcification of human aortic VSMCs (HASMCs) were investigated. RCN2 was measured in femoropopliteal artery plaque specimens from 6 peripheral arterial disease (PAD) patients with chronic kidney disease (CKD) and 6 PAD patients without CKD. RCN2 protein and mRNA expression were assessed in the high phosphate-induced aortic rings culture ex vivo model. In vitro calcification assays and molecular mechanism studies were performed in HASMCs. Immunohistochemical staining results revealed increased RCN2 expression in the calcified plaques of femoral arteries of patients with CKD and in a high phosphate-induced aortic culture ex vivo model. RCN2 promoted HASMCs osteoge...Continue Reading

References

Sep 26, 2008·Clinical Journal of the American Society of Nephrology : CJASN·Kerstin Amann
Mar 5, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Bent Honoré
Jun 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Ani ManichaikulWeibin Shi
Jul 15, 2011·Cytokine & Growth Factor Reviews·Krzysztof SikorskiHans A R Bluyssen
Oct 9, 2012·The Journal of Clinical Investigation·Maliheh Nazari-JahantighAndreas Schober
Jan 18, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Yuanyuan WeiAndreas Schober
May 16, 2013·Investigative Ophthalmology & Visual Science·Thelma EscobarCharles E Egwuagu
Jun 27, 2013·Endocrinology·Xiao-Bo LiaoLing-Qing Yuan
Aug 12, 2014·Biochemical and Biophysical Research Communications·Kyunghee LeeDaewon Jeong
Mar 5, 2015·Journal of Cellular Biochemistry·Yoshinori KakutaniMasaaki Inaba
Mar 19, 2015·Cardiovascular Research·Jochen DutzmannDaniel G Sedding
Dec 15, 2017·BioMed Research International·Dimitry A ChistiakovAlexander N Orekhov
May 23, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yonglun KongJianyun Yan
Oct 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Lipei LiuYuanli Chen
Feb 2, 2019·Pflügers Archiv : European journal of physiology·Nadeshda SchelskiIoana Alesutan
Apr 4, 2019·American Journal of Physiology. Renal Physiology·Jing LiWeibin Shi

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