Osteogenic Differentiation Modulates the Cytokine, Chemokine, and Growth Factor Profile of ASCs and SHED

International Journal of Molecular Sciences
F MussanoLuca Munaron

Abstract

Great efforts have been made to improve bone regeneration techniques owing to a growing variety of sources of stem cells suitable for autologous transplants. Specifically, adipose-derived stem cells (ASCs) and stems cells from human exfoliated deciduous teeth (SHED) hold great potential for bone tissue engineering and cell therapy. After a preliminary characterization of the main biomolecules ASCs and SHED released in their conditioned media, cells were kept both in normal and osteo-inducing conditions. Conventional assays were performed to prove their osteogenic potential such as quantitative real-time polymerase chain reaction (qRT-PCR) (for RUNX-2, collagen type I, osteopontin and osteonectin), alkaline phosphatase activity, osteocalcin production, and von Kossa staining. Conditioned media were tested again after the osteogenic induction and compared to maintaining condition both at base line and after 14 days of culture. The osteogenic condition inhibited the release of all the biomolecules, with the exception, concerning SHED, of growth-regulated alpha protein precursor (GROα), and, to a lesser extent, interleukin (IL)-8. In conclusion, our data support that undifferentiated ASCs and SHED may be preferable to committed one...Continue Reading

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Citations

Dec 12, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Giacomina BrunettiIlaria Roato
Nov 22, 2019·Clinical Oral Investigations·Luigi CanulloFederico Mussano
Mar 15, 2020·International Journal of Molecular Sciences·Luigi CanulloFederico Mussano
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Jan 11, 2020·Frontiers in Cell and Developmental Biology·Katiúcia Batista Silva PaivaAriadne Letra
Sep 11, 2020·Bulletin of Experimental Biology and Medicine·L A PokrovskayaE S Gnezdyukova
Oct 21, 2020·Journal of Clinical Medicine·Jeremie D OliverMichael S Hu

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
flow cytometry
Assay
PCR
Protein Assay
light microscopy

Software Mentioned

Excel
CellQuest
Plex
GraphPad Prism
Assay Design Center Roche Science
Bio
GraphPad Prism6
FlowLogic
Universal Probe Library

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