Osteogenic differentiation of fibroblast-like synovial cells in rheumatoid arthritis is induced by microRNA-218 through a ROBO/Slit pathway

Arthritis Research & Therapy
Naoki IwamotoAtsushi Kawakami

Abstract

Fibroblast-like synovial cells (FLS) have multilineage differentiation potential including osteoblasts. We aimed to investigate the role of microRNAs during the osteogenic differentiation of rheumatoid arthritis (RA)-FLS. RA-FLS were differentiated in osteogenic medium for 21 days. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and Alizarin Red staining. MicroRNA (miRNA) array analysis was performed to investigate the differentially expressed miRNAs during osteogenic differentiation. Expression of miR-218-5p (miR-218) during the osteogenic differentiation was determined by quantitative real-time PCR. Transfections with an miR-218 precursor and inhibitor were used to confirm the targets of miR-218 and to analyze the ability of miR-218 to induce osteogenic differentiation. Secreted Dickkopf-1 (DKK1) from FLS transfected with miR-218 precursor/inhibitor or roundabout 1 (ROBO1) knockdown FLS established using ROBO1-small interfering RNA (siRNA) were measured by ELISA. The miRNA array revealed that 12 miRNAs were upregulated and 24 miRNAs were downregulated after osteogenic differentiation. We observed that the level of miR-218 rose in the early phase of osteogenic differentiation and then decreased....Continue Reading

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Citations

May 27, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Riikka E MäkitieOuti Mäkitie
Oct 21, 2020·Clinical and Experimental Medicine·Yi ZhangXuyan Yang
Jan 6, 2021·Biomolecules·Mélanie AuréalFabienne Coury
Feb 10, 2021·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Ming ChenWei Wei
Apr 16, 2021·Advanced Science·Qian-Qian WanKai Jiao
Jun 7, 2020·Combinatorial Chemistry & High Throughput Screening·Fataneh TavasolianAmirhossein Sahebkar

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Datasets Mentioned

BETA
GSE
111946

Methods Mentioned

BETA
transfection
PCR
ELISA

Software Mentioned

TargetScan
Micro inspector
PITA
RNA hybrid
microT
Mir Target2
Pic Tar
miRanda
DIANA
GraphPad Prism

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