Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum

Stem Cell Research & Therapy
Eeva CastrénMatti Korhonen

Abstract

Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infection. Replacement of the commonly used fetal calf serum (FCS) with human platelet lysate and plasma (PLP) to support cell growth may reduce some of these risks. Altered media could, however, influence stem cell differentiation and we address this experimentally. We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified. Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the mat...Continue Reading

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Citations

Oct 28, 2016·Journal of Biomedical Materials Research. Part a·Tao WuBin Shi
Mar 30, 2017·Cold Spring Harbor Perspectives in Medicine·Johnny LamAntonios G Mikos
Dec 7, 2017·Brazilian Dental Journal·Luiz A ChisiniFlávio F Demarco
May 13, 2018·Stem Cell Research & Therapy·Yueh-Hsun Kevin YangGilda A Barabino
Apr 1, 2020·International Journal of Molecular Sciences·Terezia OkajcekovaHenrieta Skovierova
Nov 5, 2019·Cellular and Molecular Life Sciences : CMLS·Carlos Godoy-ParejoGuokai Chen
Sep 9, 2019·ACS Biomaterials Science & Engineering·Sevde AltuntasMansoor Amiji
Aug 15, 2021·Acta Biomaterialia·Juan Shong KhawSarah H Cartmell
Apr 9, 2018·ACS Biomaterials Science & Engineering·Hossein TavassoliShahin Bonakdar

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Methods Mentioned

BETA
FCS
PCR
flow cytometry

Software Mentioned

ImageJ
Adobe Photoshop

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