Osteogenic differentiation of stem cells derived from menstrual blood versus bone marrow in the presence of human platelet releasate.

Tissue Engineering. Part a
Saeedeh DarziSomaieh Kazemnejad

Abstract

In recent decades, stem cell therapy has been introduced as a novel therapeutic approach for patients suffering from bone disorders. Recently, menstrual blood has been identified as an easily accessible and recycled stem cell source. However, the osteogenic differentiation capacity of menstrual blood-derived stem cells (MenSCs) compared with other adult stem cells remained unsolved. The aim of this study was to investigate the osteogenic differentiation capacity of MenSCs compared to bone marrow-derived mesenchymal stem cells (BMSCs) in the presence of human platelet releasate (HPR). Our results showed that MenSCs were strongly positive for mesenchymal and negative for hematopoietic stem cell markers in a similar manner to BMSCs. In contrary to BMSCs, MenSCs exhibited marked expression of OCT-4 and a significantly higher proliferative capacity. Mineralization, as judged by alizarin red staining, was more pronounced in differentiated BMSCs than in differentiated MenSCs in an osteogenic medium fortified with fetal bovine serum (FBS). However, FBS substitution with HPR in a differentiation medium resulted in typical impact on intensity of MenSC mineralization. The results of semiquantitative reverse transcription-polymerase chain ...Continue Reading

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Citations

Nov 11, 2015·Human Reproduction Update·Caroline E GargettJames A Deane
Mar 19, 2013·Journal of Tissue Engineering and Regenerative Medicine·Sayeh KhanjaniSomaieh Kazemnejad
Sep 16, 2015·The Journal of Obstetrics and Gynaecology Research·Samira HosseiniAmir-Hassan Zarnani
Jan 22, 2014·Cell Biology International·Fereshteh AzediNiknam Lakpour
Jan 19, 2019·Journal of Tissue Engineering and Regenerative Medicine·Razieh DalirfardoueiElahe Mahdipour
Apr 24, 2015·Molecular Biotechnology·Sahba MobiniSomaieh Kazemnejad
Jun 3, 2020·The Journal of Obstetrics and Gynaecology Research·Sedighe EsmaeilzadehMohammad Ghasemzadeh-Hasankolaei
Jul 12, 2019·Scientific Reports·Mohammad-Reza ShokriAmir-Hassan Zarnani
Oct 24, 2018·International Journal of Molecular Sciences·Nicola TempestDharani K Hapangama
Aug 4, 2020·Frontiers in Cell and Developmental Biology·Mahmood BozorgmehrCaroline E Gargett
Dec 17, 2016·Molecular Biology Reports·Fereshteh AzediShaghayegh Arasteh
Nov 23, 2018·Stem Cell Research & Therapy·Haining LvHuidong Jia
Feb 8, 2021·Journal of Reproductive Immunology·Alireza GhanavatinejadAmir-Hassan Zarnani
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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
PCR
flow cytometry
flow cytometery

Software Mentioned

AlphaEase
SPSS

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