Osteogenic growth peptide promotes osteogenic differentiation of mesenchymal stem cells mediated by LncRNA AK141205-induced upregulation of CXCL13

Biochemical and Biophysical Research Communications
Haiqing LiDongdong Zhang

Abstract

The aim of present study was to characterize long non-coding RNA (lncRNA) AK141205 as a cellular regulator of osteogenic differentiation of mice mesenchymal stem cells (MSCs) towards osteogenic growth peptide (OGP) stimulation. Mice MSCs cells were isolated, transfected with si-AK141205, pcDNA-AK141205 or control, and stimulated with OGP. The AK141205, CXC chemokine ligand-13 (CXCL13), and osteogenic differentiation-associated parameters were determined by western blotting or quantitative RT-PCR. To determine the role of AK141205/CXCL13 in SMCs osteogenic differentiation, SMCs subjected to co-transfection of pcDNA-AK141205 and si-CXCL13 or si-AK141205 and pcDNA-CXCL13, and were submitted for osteogenic differentiation-associated parameters analyses. The results showed that stimulation of SMCs with OGP induced upregulation of both AK141205 and CXCL13, and osteogenic differentiation of MSCs. Transfection of si-AK141205 partly suppressed OGP-induced formation of calcium salt nodules, alkaline phosphatase (ALP) activity and osteogenic differentiation-associated gene expression, suggesting key regulatory role of AK141205. Analysis of CXCL13 expression in SMCs(pcDNA-AK141205) revealed that AK141205 positively promoted CXCL13 expressi...Continue Reading

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Citations

Oct 4, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Boran CaoWenbo Wang
Nov 24, 2016·International Journal of Molecular Sciences·Suzane C PigossiRaquel M Scarel-Caminaga
Apr 3, 2019·Bone Research·Andreia Machado SilvaMaria Inês Almeida
Mar 28, 2019·Bone & Joint Research·J ZhangF Guo
Apr 6, 2019·World Journal of Stem Cells·Zhong-Yu XieHui-Yong Shen
Dec 30, 2020·International Journal of Biological Macromolecules·K GomathiN Selvamurugan
Feb 24, 2021·BioFactors·Sanam DolatiSarvin Sanaie
Jul 27, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Samadian
Jul 6, 2021·Biochemical Pharmacology·Tianhong ChenJingfeng Li
Aug 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardAfshin Taheriazam
May 3, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cheng JuZhiping Zhang

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