Osteogenic markers are reduced in bone-marrow mesenchymal cells and femoral bone of young spontaneously hypertensive rats

Life Sciences
Thamine Landim de BarrosSandra Helena Penha Oliveira

Abstract

Spontaneously hypertensive rats (SHR) and normotensive rats (W) has significant changes in bone metabolism. The purpose of this study was to investigate whether, the genetic predisposition, is sufficient to induce changes in the osteoblast differentiation and osteogenic markers in the BMSCs or in the femoral bone. For this we use young SHR rats without hypertension, but, with genetic predisposition in compared with young W. BMSCs were cultured in a proliferation medium (MEM) or osteogenic medium. Osteogenic differentiation was analyzed by proliferation, total protein, alkaline phosphatase, mineralization, and the mRNA expression of RUNX-2, β-cathenin, osterix, bone morphogenetic protein-2(BMP-2), osteocalcin (OCN), bone sialoprotein (BSP), collagen type I (Col I), and osteopontin (OPN). Osteoblast differentiation in SHR BMSCs (SHRC) had an increased proliferation compared with W BMSCs (WC). After osteogenic induction, there was greater reduction in proliferation in SHR (SHROM) than in W, in the same condition (WOM). On day 7, although no significant difference in the ALP activity was observed between SHROM and WOM, poor mineralization and osteoblast differentiation was noted in SHROM. The Osterix and β-catenin are involved in t...Continue Reading

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Citations

Feb 18, 2017·Scientific Reports·Monique Patricio SingulaniRita Cássia Menegati Dornelles
Oct 27, 2017·Journal of Applied Oral Science : Revista FOB·Roberto HollandCarlos Estrela
Aug 25, 2019·Scientific Reports·Wacharaporn TiyasatkulkovitNarattaphol Charoenphandhu
Dec 29, 2020·Frontiers in Pharmacology·Victor Gustavo Balera BritoSandra Helena Penha Oliveira

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