Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells

Journal of Tissue Engineering
Aman S ChahalHanna Tiainen

Abstract

Synthetic hydrogel-amorphous calcium phosphate composites are promising candidates to substitute biologically sourced scaffolds for bone repair. While the hydrogel matrix serves as a template for stem cell colonisation, amorphous calcium phosphate s provide mechanical integrity with the potential to stimulate osteogenic differentiation. Here, we utilise composites of poly(ethylene glycol)-based hydrogels and differently stabilised amorphous calcium phosphate to investigate potential effects on attachment and osteogenic differentiation of human mesenchymal stem cells. We found that functionalisation with integrin binding motifs in the form of RGD tripeptide was necessary to allow adhesion of large numbers of cells in spread morphology. Slow dissolution of amorphous calcium phosphate mineral in the scaffolds over at least 21 days was observed, resulting in the release of calcium and zinc ions into the cell culture medium. While we qualitatively observed an increasingly mineralised extracellular matrix along with calcium deposition in the presence of amorphous calcium phosphate-loaded scaffolds, we did not observe significant changes in the expression of selected osteogenic markers.

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Citations

Jan 9, 2021·Frontiers in Cell and Developmental Biology·Hao ZhuHåvard Jostein Haugen
Mar 11, 2021·Biomedical Materials·Agnieszka PiegatLiliana Liverani
May 6, 2021·Advanced Healthcare Materials·Chang XieHongwei Ouyang
Jun 10, 2021·Science and Technology of Advanced Materials·Hisashi OzakiOsamu Suzuki
Jun 17, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J GirónP Pranke
Oct 27, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kun LuoYi Zuo
Nov 18, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bing GuoYue He

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Methods Mentioned

BETA
atomic absorption spectroscopy
scanning electron microscopy
rheology
rheometry
confocal microscopy

Software Mentioned

SigmaPlot
xPONENT

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