Osteogenic protein 1 in synovial fluid from patients with rheumatoid arthritis or osteoarthritis: relationship with disease and levels of hyaluronan and antigenic keratan sulfate.

Arthritis Research & Therapy
S ChubinskayaJoel A Block

Abstract

The measurement of body fluid levels of biochemical markers in joint tissues has begun to provide clinically useful information. Synovial fluid (SF) plays an important role in articular joint lubrication, nutrition, and metabolism of cartilage and other connective tissues within the joint. The purpose of our study was to identify and characterize osteogenic protein 1 (OP-1) in SF from patients with rheumatoid arthritis (RA) or with osteoarthritis (OA) and to correlate levels of OP-1 with those of hyaluronan (HA) and antigenic keratan sulfate (AgKS). SF was aspirated from the knees of patients with either RA or OA and from the knees of asymptomatic organ donors with no documented history of joint disease. The presence of detectable OP-1 in SF was demonstrated by western blots with specific anti-pro-OP-1 and anti-mature OP-1 antibodies. Measurement of levels of OP-1, HA and AgKS was performed using ELISAs. OP-1 was identified in human SF in two forms, pro-OP-1 and active (mature) OP-1--mature OP-1 being detected only in SF from OA patients and RA patients. Levels of OP-1 and HA were higher in RA patients than in OA patients and asymptomatic donors, while the level of AgKS was highest in SF from asymptomatic donors. Statistically ...Continue Reading

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Citations

Aug 10, 2007·International Orthopaedics·Susan ChubinskayaDavid C Rueger
Mar 21, 2009·International Orthopaedics·Sittisak HonsawekSrihatach Ngarmukos
Mar 3, 2017·Brain, Behavior, and Immunity·Herena EixarchCarmen Espejo
May 28, 2011·The American Journal of Sports Medicine·Hagen SchmalAlexander T Mehlhorn
Jul 25, 2008·Growth Factors·Susan ChubinskayaDavid C Rueger
Jul 11, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jonathan B AlbiliaSean A F Peel
May 8, 2020·Molecular Medicine Reports·Yubiao ZhangHaohuan Li

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Methods Mentioned

BETA
ELISA

Software Mentioned

Prism
GraphPad
Quantity One

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