Osteoinductive LIM mineralization protein-1 suppresses activation of NF-kappaB and selectively regulates MAPK pathways in pre-osteoclasts.

Bone
Hui LiuLouisa Titus

Abstract

LIM mineralization protein-1 (LMP-1) is an intracellular regulator of bone formation and has been shown to be osteoinductive in vitro and in vivo. The effect of LMP-1 on other aspects of bone homeostasis has not been previously studied. In a pilot study we observed that LMP-1 decreased nitric oxide (NO) production in pre-osteoclasts. Here we report a new anti-inflammatory effect of LMP-1 and define its mechanism of action in lipopolysaccharide (LPS)-stimulated RAW 264.7 pre-osteoclasts. We found that LMP-1 significantly inhibited LPS-induced NO production. LMP-1 also effectively inhibited the expression of inducible nitric oxide synthase (iNOS), potently suppressed the transcriptional activity and nuclear translocation of nuclear factor kappa B (NF-kappaB), and prevented the phosphorylation of inhibitor of kappa B (IkappaB). Interestingly, LMP-1 had no effect on Receptor-Activator of Nuclear Factor B Ligand (RANKL)-induced activation of NF-kappaB. Furthermore, LMP-1 had no effect on the LPS-induced phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), whereas it did attenuate the phosphorylation of c-Jun NH2-terminal kinase (JNK) while enhancing phosphorylation of p38 mitogen-activated protein kinases (p38 MAPK...Continue Reading

References

Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Jun 4, 1999·Progress in Biophysics and Molecular Biology·M H Cobb
Nov 11, 1999·Oncogene·F Mercurio, A M Manning
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·R J van't HofS H Ralston
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·M Becker-HapakS F Dowdy
Feb 19, 2002·Current Opinion in Biotechnology·Jehangir S Wadia, Steven F Dowdy
Sep 1, 2004·Bone·Yunshan LiuScott D Boden
Oct 7, 2004·European Journal of Pharmacology·Hartmut KleinertPetra M Schwarz
Aug 31, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Aaron Schindeler, David G Little
Jul 3, 2007·Arthritis Research & Therapy·David R Haynes
Mar 13, 2009·American Journal of Physiology. Cell Physiology·Shao-Chun LuShwu-Fen Chang
May 16, 2009·The Journal of Endocrinology·R Hardy, M S Cooper
Jun 30, 2009·The American Journal of Medicine·Tauseef AliMary Beth Humphrey

❮ Previous
Next ❯

Citations

Apr 26, 2014·International Journal of Molecular Sciences·Huiwen LiuKai Cao
Feb 14, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hehai PanHui Liu
Sep 27, 2016·International Journal of Molecular Medicine·Young-Chang ChoSayeon Cho
Jan 20, 2016·Molecular Medicine Reports·Young-Chang Cho, Sayeon Cho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.