Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11- or gentamicin-containing calcium phosphate cement

The Journal of Antimicrobial Chemotherapy
Hein P StallmannPaul I J M Wuisman

Abstract

The efficacy of prophylactic treatment with human lactoferrin 1-11 (hLF1-11), a broad-spectrum antimicrobial peptide, was studied in a rabbit model of femur infection. Calcium phosphate cement with 50 mg/g hLF1-11 or gentamicin was injected into the femoral canal, after inoculation with Staphylococcus aureus. Three weeks later, slices of the proximal femora were sawn for quantitative bacterial culture and histology. Treatment with hLF1-11 (P<0.038) or gentamicin (P<0.008) caused a reduction of cfu compared with the untreated control rabbits. The number of sterile cultures was higher in hLF1-11- (3/7) and gentamicin- (5/6) treated animals than in controls (1/7). Radiological and histological analysis showed early bone ingrowth into the cement cracks, and only moderate pathological changes in rabbits with positive cultures. Local prophylaxis with hLF1-11 effectively reduced development of osteomyelitis in a rabbit model, but gentamicin resulted in a larger number of sterile femora.

Citations

Sep 9, 2009·BMC Medicine·Walter J F M van der VeldenJ Peter Donnelly
Feb 9, 2012·Advanced Drug Delivery Reviews·Maria-Pau GinebraEdgar B Montufar
Jul 4, 2007·Transplant Infectious Disease : an Official Journal of the Transplantation Society·W J F M van der VeldenJ P Donnelly
Jan 25, 2012·Journal of Applied Microbiology·A D van StadenL M T Dicks
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·S JegatheeswaranM Sundrarajan
Jun 15, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natascia BruniFranco Dosio
Aug 9, 2016·Advanced Drug Delivery Reviews·Danir Fanisovich Bayramov, Jennifer Ann Neff
Nov 1, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hein P StallmannPaul I J M Wuisman
Apr 15, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Leah H CobbLauren B Priddy
Feb 13, 2018·Experimental and Therapeutic Medicine·Lijun HuoJia Liu
May 19, 2017·Tissue Engineering and Regenerative Medicine·Tomasz SzponderAnna Smolira
May 15, 2008·Biomacromolecules·Helen F ChuangPaula T Hammond
Apr 3, 2012·Journal of Applied Biomaterials & Functional Materials·Anton D Van Staden, Leon M T Dicks
Aug 11, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Brinta BhattacharjeeJayanta Haldar
Sep 17, 2020·Current Drug Delivery·Marina GallarateSimona Sapino

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